711652

antibody from Invitrogen Antibodies

Targeting: CALCR CTR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711652

Provider

Invitrogen Antibodies

Product name

Calcitonin Receptor Recombinant Polyclonal Antibody (1HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Horse, Dog.

Antibody clone number

1HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Calcitonin Receptor (CALCR) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-CALCR Recombinant Rabbit Polyclonal Antibody (Product # 711652 at a concentration of 1 µg/mL). A broad band from 70-80 kDa corresponding to CALCR was observed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Calcitonin Receptor (CALCR) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-CALCR Recombinant Rabbit Polyclonal Antibody (Product # 711652 at a concentration of 1 µg/mL). A broad band from 70-80 kDa corresponding to CALCR was observed.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with Calcitonin Receptor (CALCR) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-CALCR Recombinant Rabbit Polyclonal Antibody (Product # 711652 at a concentration of 1 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells.