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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-25594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calcitonin Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calcitonin Receptor in lysate from MCF-7, A431 cell line. Samples were incubated with Calcitonin Receptor polyclonal antibody (Product # PA5-25594) using a dilution of 1:1,000 followed by goat anti-rabbit (HRP) at a dilution of 1:5,000. Lysate at 35 µg per lane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using a CALCR polyclonal antibody (Product # PA5-25594) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Calcitonin Receptor in MCF-7 cells. Samples were incubated in Calcitonin Receptor polyclonal antibody (Product # PA5-25594) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HL-60 cells using a CALCR polyclonal antibody (Product # PA5-25594) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Calcitonin Receptor in HL-60 cells (right histogram). Samples were incubated with Calcitonin Receptor polyclonal antibody (Product # PA5-25594) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
