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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [2]
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- Product number
- MAB4614 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Calcitonin R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Calcitonin R. Stains human Calcitonin R transfectants but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_001733- Isotype
- IgG
- Antibody clone number
- 462802
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Extracellular loops 2 and 3 of the calcitonin receptor selectively modify agonist binding and efficacy.
Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells.
Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media.
Dal Maso E, Zhu Y, Pham V, Reynolds CA, Deganutti G, Hick CA, Yang D, Christopoulos A, Hay DL, Wang MW, Sexton PM, Furness SGB, Wootten D
Biochemical pharmacology 2018 Apr;150:214-244
Biochemical pharmacology 2018 Apr;150:214-244
Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
Kasonga AE, Deepak V, Kruger MC, Coetzee M
PloS one 2015;10(4):e0125145
PloS one 2015;10(4):e0125145
Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells.
Costa-Rodrigues J, Martins EG, Fernandes MH
Bone 2012 Jul;51(1):17-27
Bone 2012 Jul;51(1):17-27
Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media.
Costa-Rodrigues J, Teixeira CA, Sampaio P, Fernandes MH
Journal of cellular biochemistry 2010 Jan 1;109(1):205-16
Journal of cellular biochemistry 2010 Jan 1;109(1):205-16
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Calcitonin R in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Calcitonin R Specificity is Shown by Flow Cytometry in Knockout Cell Line. Calcitonin R knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB4614, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Calcitonin R knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
