Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-52345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QDTVELREHH WVPRKAFLDN GPKTINQIRQ DAVKDLGVFI PAPMAQGMRS DFFLEGPFMP PRMKMDRDPL GGLADMFGQM PGSGIGTGPG VIQDRFSPTM GRHRSNQLFN GHGGHIMPPT QSQFGEMGGK FMKSQGLSQL YH
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAP5 in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using a DAP5 Polyclonal Antibody (Product # PA5-52345). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAP5 using DAP5 Polyclonal Antibody (Product # PA5-52345) (A) shows similar pattern to an independent DAP5 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAP5 in mouse cell line NIH-3T3 and rat cell line NBT-II using a DAP5 Polyclonal Antibody (Product # PA5-52345).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DAP5 in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a DAP5 Polyclonal Antibody (Product # PA5-52345).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of DAP5 in human testis using DAP5 Polyclonal Antibody (Product # PA5-52345) shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAP5 in human placenta using DAP5 Polyclonal Antibody (Product # PA5-52345) shows strong cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAP5 in human skeletal muscle using DAP5 Polyclonal Antibody (Product # PA5-52345) shows no positivity in myocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DAP5 in human gastrointestinal using DAP5 Polyclonal Antibody (Product # PA5-52345) shows moderate to strong cytoplasmic positivity in glandular cells.