Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF2770 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-22R alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
IL-22 contributes to TGF-β1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Chen JP, Wu MS, Kuo SH, Liao F
PloS one 2014;9(5):e97350
PloS one 2014;9(5):e97350
IL-22 contributes to TGF-β1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.
Johnson JR, Nishioka M, Chakir J, Risse PA, Almaghlouth I, Bazarbashi AN, Plante S, Martin JG, Eidelman D, Hamid Q
Respiratory research 2013 Nov 1;14(1):118
Respiratory research 2013 Nov 1;14(1):118
IL-22 suppresses IFN-γ-mediated lung inflammation in asthmatic patients.
Pennino D, Bhavsar PK, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham SR, Cavani A, Eyerich K, Chung KF, Schmidt-Weber CB, Eyerich S
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
The Journal of allergy and clinical immunology 2013 Feb;131(2):562-70
IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
European journal of immunology 2009 Oct;39(10):2779-88
European journal of immunology 2009 Oct;39(10):2779-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- IL-10 secretion Induced by IL-22 and Neutralization by Human IL-22 R alpha 1 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically 0.2-1 µg/mL.