V3485

antibody from NSJ Bioreagents

Targeting: CD81 TAPA-1, TAPA1, TSPAN28

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Functional assay (Recommended by provider)

Antibody Data

Product number

V3485

Provider

NSJ Bioreagents

Product name

CD81 Antibody

Antibody type

Monoclonal

Description

Purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

1.3.3.22

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml

Storage

Store the CD81 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Apolipoprotein E, but Not Apolipoprotein B, Is Essential for Efficient Cell-to-Cell Transmission of Hepatitis C Virus.

Gondar V, Molina-Jiménez F, Hishiki T, García-Buey L, Koutsoudakis G, Shimotohno K, Benedicto I, Majano PL
Journal of virology 2015 Oct;89(19):9962-73

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Fibroblast-Derived Exosomes Contribute to Chemoresistance through Priming Cancer Stem Cells in Colorectal Cancer.

Hu Y, Yan C, Mu L, Huang K, Li X, Tao D, Wu Y, Qin J
PloS one 2015 May 4;10(5):e0125625

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A mutation in the human tetraspanin CD81 gene is expressed as a truncated protein but does not enable CD19 maturation and cell surface expression.

Vences-Catalán F, Kuo CC, Sagi Y, Chen H, Kela-Madar N, van Zelm MC, van Dongen JJ, Levy S
Journal of clinical immunology 2015 Apr;35(3):254-63

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Impact of single nucleotide polymorphisms in the essential HCV entry factor CD81 on HCV infectivity and neutralization.

Deest M, Westhaus S, Steinmann E, Manns MP, von Hahn T, Ciesek S
Antiviral research 2014 Jan;101:37-44

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Cell-cell contact-mediated hepatitis C virus (HCV) transfer, productive infection, and replication and their requirement for HCV receptors.

Liu Z, He JJ
Journal of virology 2013 Aug;87(15):8545-58

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Computational reconstruction of Bole1a, a representative synthetic hepatitis C virus subtype 1a genome.

Munshaw S, Bailey JR, Liu L, Osburn WO, Burke KP, Cox AL, Ray SC
Journal of virology 2012 May;86(10):5915-21

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Blocking HCV entry as potential antiviral therapy

Vercauteren K, Leroux-Roels G, Meuleman P
Future Virology 2012 June;7(6):547-561

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Hepatitis C virus induces CD81 and claudin-1 endocytosis.

Farquhar MJ, Hu K, Harris HJ, Davis C, Brimacombe CL, Fletcher SJ, Baumert TF, Rappoport JZ, Balfe P, McKeating JA
Journal of virology 2012 Apr;86(8):4305-16

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Hepatocyte-derived cultured cells with unusual cytoplasmic keratin-rich spheroid bodies.

Delavalle PY, Alsaleh K, Pillez A, Cocquerel L, Allet C, Dumont P, Loyens A, Leteurtre E, Omary MB, Dubuisson J, Rouillé Y, Wychowski C
Experimental cell research 2011 Nov 1;317(18):2683-94

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Tupaia CD81, SR-BI, claudin-1, and occludin support hepatitis C virus infection.

Tong Y, Zhu Y, Xia X, Liu Y, Feng Y, Hua X, Chen Z, Ding H, Gao L, Wang Y, Feitelson MA, Zhao P, Qi ZT
Journal of virology 2011 Mar;85(6):2793-802

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Interacting regions of CD81 and two of its partners, EWI-2 and EWI-2wint, and their effect on hepatitis C virus infection.

Montpellier C, Tews BA, Poitrimole J, Rocha-Perugini V, D'Arienzo V, Potel J, Zhang XA, Rubinstein E, Dubuisson J, Cocquerel L
The Journal of biological chemistry 2011 Apr 22;286(16):13954-65

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Hepatitis C virus hypervariable region 1 modulates receptor interactions, conceals the CD81 binding site, and protects conserved neutralizing epitopes.

Bankwitz D, Steinmann E, Bitzegeio J, Ciesek S, Friesland M, Herrmann E, Zeisel MB, Baumert TF, Keck ZY, Foung SK, Pécheur EI, Pietschmann T
Journal of virology 2010 Jun;84(11):5751-63

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Adaptation of hepatitis C virus to mouse CD81 permits infection of mouse cells in the absence of human entry factors.

Bitzegeio J, Bankwitz D, Hueging K, Haid S, Brohm C, Zeisel MB, Herrmann E, Iken M, Ott M, Baumert TF, Pietschmann T
PLoS pathogens 2010 Jul 1;6:e1000978

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Mouse-specific residues of claudin-1 limit hepatitis C virus genotype 2a infection in a human hepatocyte cell line.

Haid S, Windisch MP, Bartenschlager R, Pietschmann T
Journal of virology 2010 Jan;84(2):964-75

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Role of scavenger receptor class B type I in hepatitis C virus entry: kinetics and molecular determinants.

Catanese MT, Ansuini H, Graziani R, Huby T, Moreau M, Ball JK, Paonessa G, Rice CM, Cortese R, Vitelli A, Nicosia A
Journal of virology 2010 Jan;84(1):34-43

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The association of CD81 with tetraspanin-enriched microdomains is not essential for Hepatitis C virus entry.

Rocha-Perugini V, Lavie M, Delgrange D, Canton J, Pillez A, Potel J, Lecoeur C, Rubinstein E, Dubuisson J, Wychowski C, Cocquerel L
BMC microbiology 2009 May 28;9:111

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Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.

Rohlena J, Volger OL, van Buul JD, Hekking LH, van Gils JM, Bonta PI, Fontijn RD, Post JA, Hordijk PL, Horrevoets AJ
Cardiovascular research 2009 Jan 1;81(1):187-96

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Targeting the tetraspanin CD81 blocks monocyte transmigration and ameliorates EAE.

Dijkstra S, Kooij G, Verbeek R, van der Pol SM, Amor S, Geisert EE Jr, Dijkstra CD, van Noort JM, Vries HE
Neurobiology of disease 2008 Sep;31(3):413-21

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The single subunit transmembrane E3 ligase gene related to anergy in lymphocytes (GRAIL) captures and then ubiquitinates transmembrane proteins across the cell membrane.

Lineberry N, Su L, Soares L, Fathman CG
The Journal of biological chemistry 2008 Oct 17;283(42):28497-505

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Anti-CD81 antibodies can prevent a hepatitis C virus infection in vivo.

Meuleman P, Hesselgesser J, Paulson M, Vanwolleghem T, Desombere I, Reiser H, Leroux-Roels G
Hepatology (Baltimore, Md.) 2008 Dec;48(6):1761-8

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The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells.

Koutsoudakis G, Herrmann E, Kallis S, Bartenschlager R, Pietschmann T
Journal of virology 2007 Jan;81(2):588-98

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High-avidity monoclonal antibodies against the human scavenger class B type I receptor efficiently block hepatitis C virus infection in the presence of high-density lipoprotein.

Catanese MT, Graziani R, von Hahn T, Moreau M, Huby T, Paonessa G, Santini C, Luzzago A, Rice CM, Cortese R, Vitelli A, Nicosia A
Journal of virology 2007 Aug;81(15):8063-71

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Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry.

Evans MJ, von Hahn T, Tscherne DM, Syder AJ, Panis M, Wölk B, Hatziioannou T, McKeating JA, Bieniasz PD, Rice CM
Nature 2007 Apr 12;446(7137):801-5

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lymph node with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.