Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [6]
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Validation data
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- Product number
- NB100-65805 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65805, RRID:AB_2076378
- Product name
- Mouse Monoclonal CD81 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Goat, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.
Multiplexed profiling of single-cell extracellular vesicles secretion.
Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.
Correlation between Cancerous Exosomes and Protein Markers Based on Surface-Enhanced Raman Spectroscopy (SERS) and Principal Component Analysis (PCA).
A rapid, automated surface protein profiling of single circulating exosomes in human blood.
Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.
Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.
Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
Roma-Rodrigues C, Fernandes AR, Baptista PV
International journal of nanomedicine 2019;14:6843-6854
International journal of nanomedicine 2019;14:6843-6854
Multiplexed profiling of single-cell extracellular vesicles secretion.
Ji Y, Qi D, Li L, Su H, Li X, Luo Y, Sun B, Zhang F, Lin B, Liu T, Lu Y
Proceedings of the National Academy of Sciences of the United States of America 2019 Mar 26;116(13):5979-5984
Proceedings of the National Academy of Sciences of the United States of America 2019 Mar 26;116(13):5979-5984
Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.
Otake K, Kamiguchi H, Hirozane Y
BMC medical genomics 2019 Jan 10;12(1):7
BMC medical genomics 2019 Jan 10;12(1):7
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.
Ren W, Hou J, Yang C, Wang H, Wu S, Wu Y, Zhao X, Lu C
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62
Correlation between Cancerous Exosomes and Protein Markers Based on Surface-Enhanced Raman Spectroscopy (SERS) and Principal Component Analysis (PCA).
Shin H, Jeong H, Park J, Hong S, Choi Y
ACS sensors 2018 Dec 28;3(12):2637-2643
ACS sensors 2018 Dec 28;3(12):2637-2643
A rapid, automated surface protein profiling of single circulating exosomes in human blood.
Kibria G, Ramos EK, Lee KE, Bedoyan S, Huang S, Samaeekia R, Athman JJ, Harding CV, Lötvall J, Harris L, Thompson CL, Liu H
Scientific reports 2016 Nov 7;6:36502
Scientific reports 2016 Nov 7;6:36502
Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.
Tian ZF, Shen H, Fu XH, Chen YC, Blum HE, Baumert TF, Zhao XP
World journal of gastroenterology 2009 Jan 14;15(2):240-4
World journal of gastroenterology 2009 Jan 14;15(2):240-4
Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.
Tian ZF, Shen H, Fu XH, Chen YC, Blum HE, Baumert TF, Zhao XP
World journal of gastroenterology 2009 Jan 14;15(2):240-4
World journal of gastroenterology 2009 Jan 14;15(2):240-4
Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
Flint M, Maidens C, Loomis-Price LD, Shotton C, Dubuisson J, Monk P, Higginbottom A, Levy S, McKeating JA
Journal of virology 1999 Aug;73(8):6235-44
Journal of virology 1999 Aug;73(8):6235-44
Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
Flint M, Maidens C, Loomis-Price LD, Shotton C, Dubuisson J, Monk P, Higginbottom A, Levy S, McKeating JA
Journal of virology 1999 Aug;73(8):6235-44
Journal of virology 1999 Aug;73(8):6235-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.