PA5-29759

antibody from Invitrogen Antibodies

Targeting: RLBP1 CRALBP

Antibody data

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Validation data

Product number: PA5-29759 - Provider product page
Provider: Invitrogen Antibodies
Product name: CRALBP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2, mouse eye. Predicted reactivity: Mouse (91%), Rat (91%), Xenopus laevis (82%), Chicken (86%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.41 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RLBP1 protein structure - PA5-29759 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CRALBP using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CRALBP polyclonal antibody (Product # PA5-29759) at a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of CRALBP was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CRALBP Polyclonal Antibody (Product # PA5-29759) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CRALBP Polyclonal Antibody (Product # PA5-29759). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CRALBP Polyclonal Antibody (Product # PA5-29759) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CRALBP Rabbit Polyclonal Antibody (Product # PA5-29759) and a 35kDa band corresponding to CRALBP was observed across tissues tested except Mouse Liver, Rat Liver, Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported negative for CRALBP expression. Tissue extracts (30 µg lysate) of Mouse Retina (Lane 1), Mouse Liver (Lane 2), Rat Liver (Lane 3), Mouse Skeletal Muscle (Lane 4) and Rat Skeletal Muscle (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).