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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-41616 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRHL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: EEVFDALMLK TPDLKGLRNA ISEKYGFPEE NIYKVYKKCK RGILVNMDNN Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 100%; Rat: 93%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche.
Salazar VS, Capelo LP, Cantù C, Zimmerli D, Gosalia N, Pregizer S, Cox K, Ohte S, Feigenson M, Gamer L, Nyman JS, Carey DJ, Economides A, Basler K, Rosen V
eLife 2019 Feb 8;8
eLife 2019 Feb 8;8
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 12. Bmp2 acts downstream of canonical Wnt pathway to specify Sp7+/Col1A1 + osteoblasts. ( a ) Cartoon summarizing regulatory analysis of Sp7 locus by chromatin immunoprecipitation of H3-acetyl-K14, Smad1, endogenous Grhl3, or transiently expressed V5-GRHL3 from chromatin of immortalized E13.5 limb bud cell cultures grown in presence of recombinant BMP2 or Noggin. ( b ) QPCR analysis revealed BMP-dependent enrichment of these proteins at a putative consensus-binding motif for Grhl3 located at a ( c ) highly conserved genomic region ~13 kb upstream of the Sp7 transcription start site. Data reflect mean +-distribution in two independent experiments.
