LS-C796581

antibody from LSBio

Targeting: FANCI FLJ10719, KIAA1794

Western blot (Enhanced validation)

Antibody Data

Product number

LS-C796581

Provider

LSBio

Product name

FANCI Antibody LS-C796581

Antibody type

Polyclonal

Description

Affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY FANCI. (Right lane) cDNA for 48 hrs and lysed

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Equivalent amounts of cell lysates and FANCI-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-FANCI monoclonal antibody(1:500`. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Equivalent amounts of cell lysates and FANCI-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-FANCI monoclonal antibody(1:500`. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts. (35ug) from different cell lines or tissues by using anti-FANCI rabbit polyclonal antibody .

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY FANCI. (Right lane) cDNA for 48 hrs and lysed