Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-60447 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60447, RRID:AB_905546
- Product name
- Rabbit Polyclonal FANCI Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair.
Colnaghi L, Jones MJ, Cotto-Rios XM, Schindler D, Hanenberg H, Huang TT
Blood 2011 Feb 17;117(7):2247-56
Blood 2011 Feb 17;117(7):2247-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FANCI Antibody [NB100-60447] - Detection of Human FANCI on HeLa whole cell lysate using NB100-60447. FANCI was also immunoprecipitated, albeit less efficiently, by another rabbit anti-FANCI antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FANCI Antibody [NB100-60447] - Detection of Human FANCI by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FANCI Antibody [NB100-60447] - Detection of human FANCI by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FANCI Antibody [NB100-60447] - Detection of human FANCI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FANCI antibody NB100-60447 used for IP at 3 ug per reaction. FANCI was also immunoprecipitated by rabbit anti-FANCI antibody from Company B. For blotting immunoprecipitated FANCI, NB100-60447 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.