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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- GTX629632 - Provider product page
- Provider
- GeneTex
- Product name
- UCHL5 / UCH37 antibody [EPR4896]
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- DDDDK tag antibody [GT1423] (GTX629632) detects DDDDK-tagged proteins by western blot.A. Untransfected 293T whole cell lysate/extract (30 ?g)B.-D. Whole cell lysate/extracts (30 ?g) from transfected 293T cells expressing different DDDDK-tagged proteins.10% SDS-PAGEDDDDK tag antibody [GT1423] (GTX629632) dilution: 1:5000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DDDDK tag antibody [GT1423] immunoprecipitates DDDK-tagged protein in IP experiments. IP Sample: 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 £gg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 £gg of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 £gg of DDDDK tag antibody [GT1423] (GTX629632) 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT1423] (GTX629632) diluted at 1 : 5000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
