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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- ABIN1881974 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5) (Center), (AA 136-164) antibody
- Antibody type
- Polyclonal
- Antigen
- This UCHL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human UCHL5.
- Description
- This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Center,AA 136-164
- Antibody clone number
- RB42329
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
Submitted references Crystal structure of the de-ubiquitinating enzyme UCH37 (human UCH-L5) catalytic domain.
Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex.
Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome.
A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes.
Nishio K, Kim SW, Kawai K, Mizushima T, Yamane T, Hamazaki J, Murata S, Tanaka K, Morimoto Y
Biochemical and biophysical research communications 2009 Dec 18;390(3):855-60
Biochemical and biophysical research communications 2009 Dec 18;390(3):855-60
Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex.
Yao T, Song L, Jin J, Cai Y, Takahashi H, Swanson SK, Washburn MP, Florens L, Conaway RC, Cohen RE, Conaway JW
Molecular cell 2008 Sep 26;31(6):909-17
Molecular cell 2008 Sep 26;31(6):909-17
Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome.
Koulich E, Li X, DeMartino GN
Molecular biology of the cell 2008 Mar;19(3):1072-82
Molecular biology of the cell 2008 Mar;19(3):1072-82
A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes.
Hamazaki J, Iemura S, Natsume T, Yashiroda H, Tanaka K, Murata S
The EMBO journal 2006 Oct 4;25(19):4524-36
The EMBO journal 2006 Oct 4;25(19):4524-36
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- UCHL5 Antibody (Center) (ABIN1881974) western blot analysis in NCI-H460 cell line lysates (35 μg/lane). This demonstrates the UCHL5 antibody detected the UCHL5 protein (arrow).
