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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-85693 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UCH37 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (83%), Xenopus laevis (90%), Pig (98%), Chicken (89%), Bovine (98%). Positive Control: 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.48 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UCH37 in whole cell extract using UCH37 polyclonal antibody (Product # PA5-85693) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UCH37 Polyclonal Antibody detects UCHL5 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with UCH37 Polyclonal Antibody (Product # PA5-85693) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of UCH37 in 293T whole cell extracts with UCH37 polyclonal antibody (Product # PA5-85693) using 5 µg of sample. Sample was then incubated with anti-Mouse IgG secondary antibody.
