PAB10076

antibody from Abnova Corporation

Targeting: MAD2L1 HSMAD2, MAD2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10076

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10076, RRID:AB_1676485

Product name

MAD2L1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MAD2L1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer.

Wu CW, Chi CW, Huang TS
World journal of gastroenterology 2004 Nov 15;10(22):3240-4

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Mad2 and p27 expression profiles in colorectal cancer and its clinical significance.

Li GQ, Zhang HF
World journal of gastroenterology 2004 Nov 1;10(21):3218-20

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A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint.

Wang RH, Yu H, Deng CX
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 7;101(49):17108-13

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using MAD2L1 polyclonal antibody (Cat # PAB10076) shows detection of a predominant band at ~24 KDa corresponding to MAD2L1 (arrowhead) present in Jurkat (Lane 1) and HeLa (Lane 2) whole cell lysates using the 800 nmchannel (green).The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide (lanes 4 and 5 respectively) which completely blocks antibody staining.Approximately 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1200.Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.Molecular weight markers were used for size comparison using the 700 nm channel (Lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.