PA5-22283

antibody from Invitrogen Antibodies

Targeting: MAD2L1 HSMAD2, MAD2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22283

Provider

Invitrogen Antibodies

Product name

MAD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (94%), Rat (95%), Xenopus laevis (81%), Chicken (87%), Rhesus Monkey (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MAD2 Polyclonal Antibody (Product # PA5-22283). Sample (30 µg of whole cell lysate). Lane A: 293T. 12% SDS PAGE. MAD2 Polyclonal Antibody (Product # PA5-22283) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Mitotic spindle assembly checkpoint protein MAD2A was achieved by transfecting HEK-293 with Mitotic spindle assembly checkpoint protein MAD2A specific siRNAs (Silencer® select Product # s8393, s8392). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Mitotic spindle assembly checkpoint protein MAD2A knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MAD2 Polyclonal Antibody (Product # PA5-22283, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Mitotic spindle assembly checkpoint protein MAD2A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MAD2 Polyclonal Antibody (Product # PA5-22283) and a 23 kDa band corresponding to Mitotic spindle assembly checkpoint protein MAD2A was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), SH-SY5Y (Lane 2), HeLa (Lane 3), A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).