Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-31311 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31311, RRID:AB_2139383
- Product name
- Rabbit Polyclonal MAD2L1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Nucleoporin Nup188 is required for chromosome alignment in mitosis.
Itoh G, Sugino S, Ikeda M, Mizuguchi M, Kanno S, Amin MA, Iemura K, Yasui A, Hirota T, Tanaka K
Cancer science 2013 Jul;104(7):871-9
Cancer science 2013 Jul;104(7):871-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAD2L1 Antibody [NBP1-31311] - A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE NBP1-31311 diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAD2L1 Antibody [NBP1-31311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAD2L1 Antibody [NBP1-31311] - MAD2L1 protein from 293T whole cell extracts using 5 ug of MAD2L1 antibody [C2C3], C-term (NBP1-31311). Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (NBP1-31311). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Paraffin-embedded rat fore brain. MAD2L1 antibody [C2C3], C-term dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAD2L1 Antibody [NBP1-31311] - Paraffin-embedded human A549 xenograft . MAD2L1 antibody [C2C3], C-term diluted at 1:250.