GTX10691

antibody from GeneTex

Targeting: MAD2L1 HSMAD2, MAD2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX10691

Provider

GeneTex

Proper citation

GeneTex Cat#GTX10691, RRID:AB_371688

Product name

MAD2L1 antibody [17D10]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex Mab anti-MAD2L1 antibody (GTX10691) shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex Mab anti-MAD2L1 antibody (GTX10691) shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ?g of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4¢XC with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye?800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye?800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.