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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-21594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, NCIN87, U2OS, HepG2, Molt4, Raji. Predicted reactivity: Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references CCAR2 controls mitotic progression through spatiotemporal regulation of Aurora B.
Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.
Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest.
TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.
Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells.
Ryu J, Kim JE
Cell death & disease 2022 Jun 7;13(6):534
Cell death & disease 2022 Jun 7;13(6):534
Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.
Kumari A, Kumar C, Pergu R, Kumar M, Mahale SP, Wasnik N, Mylavarapu SVS
The Journal of cell biology 2021 Dec 6;220(12)
The Journal of cell biology 2021 Dec 6;220(12)
Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest.
Allan LA, Skowyra A, Rogers KI, Zeller D, Clarke PR
The EMBO journal 2018 Sep 3;37(17)
The EMBO journal 2018 Sep 3;37(17)
TC Mps1 12, a novel Mps1 inhibitor, suppresses the growth of hepatocellular carcinoma cells via the accumulation of chromosomal instability.
Choi M, Min YH, Pyo J, Lee CW, Jang CY, Kim JE
British journal of pharmacology 2017 Jun;174(12):1810-1825
British journal of pharmacology 2017 Jun;174(12):1810-1825
Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells.
Choi M, Kim W, Cheon MG, Lee CW, Kim JE
Cancer letters 2015 Feb 28;357(2):591-601
Cancer letters 2015 Feb 28;357(2):591-601
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MAD2 Polyclonal Antibody (Product # PA5-21594). Sample (30 µg whole cell lysate). A: Hep G2. B: MOLT4. C: Raji. 12% SDS PAGE. MAD2 Polyclonal Antibody (Product # PA5-21594) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody (Product # PA5-21594) at 1:1,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MAD2L1 was performed in 293T whole cell extracts using 5 µg of MAD2 Polyclonal Antibody (Product # PA5-21594). Samples were transferred to a membrane and probed with MAD2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 CCAR2 deficiency induces inactivation of spindle assembly checkpoint. A , B Asynchronous A549 cells were stained with Hoechst, CREST, and anti-Mad2 or anti-BubR1 antibodies. Fluorescence intensity of kinetochore-associated Mad2 (A) or BubR1 (B) in siCCAR2-a cells was normalized to that of siCon at prometaphase. The number of prometaphase in all experiments ( n ) was presented in each graph. The first and third bars are the 25th and 75th percentiles, respectively, and the second bar is the median. * p < 0.05, significantly different from corresponding siCon cells (Mann-Whitney U test); A.U., arbitrary units; scale bar, 10 mum. C Cells were arrested at the G1/S boundary by double thymidine block and then released for 9 h. MG132 (30 muM) was treated for the last three hours to block anaphase onset. The amount of each protein was determined by western blotting.
