15816-1-AP

antibody from Proteintech Group

Targeting: PRDX1 NKEFA, PAGA

Provider product page for 15816-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 15816-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15816-1-AP, RRID:AB_2170318
Product name: PRDX1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PRDX1 antibody (Cat. #15816-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PRDX1 protein structure - 15816-1-AP shown in red.

Submitted references The YY1-KIF15-PRDX1 axis promotes gastric cancer progression by inducing mitochondrial ROS imbalance.

Li W, Wu B, Qian H, Zhang G, Wang X, Li X
Oncogene 2026 May;45(16):1437-1452

Ginsenoside Rg1 mitigates sepsis-associated acute respiratory distress syndrome by promoting autophagy through the Prdx1-PTEN/PI3K/AKT pathway.

Xue X, Huang C, Chen J, Ding W, Ren Y, Zhang W, Li Q, Yang Z, Sun Z
Phytomedicine : international journal of phytotherapy and phytopharmacology 2026 May;154:158027

The natural flavonoid dihydromyricetin targets senescent cells via PRDX2 and alleviates age-related diseases.

Xu Q, Li G, Zhang H, Jiang Z, Gao X, Li Z, Langhi Prata LGP, Kirkland JL, Zhang G, Sun Y
Nature communications 2026 Mar 6;17(1)

IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury.

Huang N, Zhang Q, Chen Y, Yu D, Liu R, Kang J, Fang X, Zhang Y, Bian H, Zhao Y, Yin Y, Zhang C, Jia Y, Chen Q, Fang Y, Li S, Li F, Jin Z, Ning B
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar 31;:e04511

Lipopolysaccharide Upregulates Neuroinflammation, Oxidative Stress Responses, and Peroxiredoxins in Depression Models.

Zhang Z, Li N, Liang M, Qin F, Qin Q, He Q, Wang K, Shi X, Jiang Y, Qin H
Brain and behavior 2026 Feb;16(2):e71231

Clinical value and molecular role of PRDXs family in prostate cancer.

Yao Y, Zheng Q, Qian C, Gu P, Zhang M, Zhang Z
Frontiers in oncology 2025;15:1713670

Coenzyme A protects against ferroptosis via CoAlation of mitochondrial thioredoxin reductase.

Lin CC, Lin YT, Chen SY, Setayeshpour Y, Chen Y, Dunn DE, Nguyen T, Mestre AA, Banerjee A, Guruprasad L, Soderblom EJ, Zhang GF, Lin CY, Filonenko V, Jeong SY, Floyd SR, Hayflick SJ, Gout I, Chi JT
The Journal of clinical investigation 2025 Oct 1;135(19)

O-GlcNAcylation of PRDX1 enhances its stability and promotes liver cancer progression via activating LRP6-mediated Wnt signaling.

Dai L, Xu Z, Fan Y, Gao W, Yuan G, He S, Mao L
Cellular signalling 2025 Nov;135:112055

Pgk1 activation restores endothelial metabolic homeostasis to alleviate vascular aging and atherosclerosis.

Lu J, Wang J, Liu Y, Zhang C, Zhang G, Ge Y, Liu L
Cardiovascular diabetology 2025 Nov 8;24(1):427

The ER thioredoxin-related transmembrane protein TMX2 controls redox-mediated tethering of ER-mitochondria contacts.

Chen J, Yap MC, Bassot A, Pascual DM, Makio T, Zimmermann J, Mast H, Bhat R, Fleury SG, Fan Y, Buzatto AZ, Moore J, Ballanyi K, Li L, Overduin M, Lemieux MJ, Lemieux H, Mancini GMS, Morgan B, Marcogliese PC, Simmen T
Cell reports 2025 Nov 25;44(11):116486

Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1.

Sun Y, Ji C, Zhang Y, Liu S, Ye Y, Li T, Wu S, Sun T, Yin H, Yan W, Jin W
Journal of cellular biochemistry 2025 Mar;126(3):e70026

Mitomycin induced the senescence of alveolar epithelial cells by targeting peroxiredoxin I.

Sun X, Wan X, Chen L, Cui Y, Chen X, Zhang J, Jiang X
Biochemical pharmacology 2025 Jun;236:116890

UGDH promotes 5-fluorouracil resistance in colorectal cancer via the ROS-activated PI3K/AKT-EEF1A2-PRDX1 pathway.

Cai J, Zhou R, Ye Y, Hao J, Luo Q, Mei H, He Z, Wang F, Yalikun A, Yu Y, Wen Y
Archives of biochemistry and biophysics 2025 Jul;769:110445

Artesunate induces ferroptosis in diffuse large B-cell lymphoma cells by targeting PRDX1 and PRDX2.

Liu X, Zeng L, Liu J, Huang Y, Yao H, Zhong J, Tan J, Gao X, Xiong D, Liu L
Cell death & disease 2025 Jul 11;16(1):513

Modulation of the thiol redox proteome by sugarcane ash-derived silica nanoparticles: insights into chronic kidney disease of unknown etiology.

Stem AD, Michel CR, Harris PS, Rogers KL, Gibb M, Roncal-Jimenez CA, Reisdorph R, Johnson RJ, Roede JR, Fritz KS, Brown JM
Particle and fibre toxicology 2025 Feb 6;22(1):3

Discovery of Natural Resorcylic Acid Lactones as Novel Potent Copper Ionophores Covalently Targeting PRDX1 to Induce Cuproptosis for Triple-Negative Breast Cancer Therapy.

Feng L, Wu TZ, Guo XR, Wang YJ, Wang XJ, Liu SX, Zhang R, Ma Y, Tan NH, Bian JL, Wang Z
ACS central science 2025 Feb 26;11(2):357-370

l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function.

Liu S, Jin Y, Zhou W, Liu J, Tu L, Zhang Y, Zhang M, Liu X, Cao Y, Guo W, Fu S
Journal of agricultural and food chemistry 2025 Feb 12;73(6):3483-3493

PFGA12 ameliorates Hypoxic-Ischemic brain injury by directly regulating PRDX1 and inhibiting ferroptosis.

Chen H, Zhao J, Kang Y, Wang S, Qin D, Yu L, Zhao Y, Zhang G, Dong X
Biochemical pharmacology 2025 Dec;242(Pt 2):117307

Augmentation of PRDX1-DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation.

Dang W, Wang X, Li H, Xu Y, Li X, Huang S, Tao H, Li X, Yang Y, Xuan L, Xiao W, Guo D, Zhang H, Wu Q, Zheng J, Shen X, Chen K, Xu H, Zhang Y, Luo C
Acta pharmaceutica Sinica. B 2025 Aug;15(8):3997-4013

PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer.

Song Y, Wang X, Sun Y, Yu N, Tian Y, Han J, Qu X, Yu X
International journal of biological sciences 2024;20(13):5070-5086

Peroxiredoxin-1 as a molecular chaperone that regulates glutathione S-transferase P1 activity and drives mutidrug resistance in ovarian cancer cells.

Fan C, Yuan S, Zhang Y, Nie Y, Xiang L, Luo T, Xi Q, Zhang Y, Gu Z, Wang P, Zhou H
Biochemistry and biophysics reports 2024 Mar;37:101639

Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome.

Chen Y, Liu Z, Chen H, Wen Y, Fan L, Luo M
Biochimica et biophysica acta. Molecular basis of disease 2024 Jun;1870(5):167182

Coenzyme A protects against ferroptosis via CoAlation of thioredoxin reductase 2.

Chi JT, Lin CC, Lin YT, Chen SY, Setayeshpour Y, Chen Y, Dunn D, Soderblom E, Zhang GF, Filonenko V, Jeong SY, Floyd S, Hayflick S, Gout I
Research square 2024 Jun 18;

Induction of Peroxiredoxin 1 by Hypoxia Promotes Cellular Autophagy and Cell Proliferation in Oral Leukoplakia via HIF-1α/BNIP3 Pathway.

Li J, Li W, Li L, Wang W, Zhang M, Tang X
Journal of molecular histology 2024 Aug;55(4):403-413

The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells.

Ma J, Huang R, Zhang H, Liu D, Dong X, Xiong Y, Xiong X, Lan D, Fu W, He H, Li J, Yin S
International journal of molecular sciences 2024 Aug 12;25(16)

Physalin H ameliorates LPS-induced acute lung injury via KEAP1/NRF2 axis.

Cai Y, Zhu J, Zhu L, Hong L, Zhang J, Kong L, Chen C, Luo J
International immunopharmacology 2024 Apr 20;131:111789

A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder.

Feng H, Deng Z, Huang Y, Liu Z, Ruan Y, Wang T, Liu J
Frontiers in immunology 2023;14:1219209

Exogenous spermidine alleviates diabetic cardiomyopathy via suppressing reactive oxygen species, endoplasmic reticulum stress, and Pannexin-1-mediated ferroptosis.

Sun J, Xu J, Liu Y, Lin Y, Wang F, Han Y, Zhang S, Gao X, Xu C, Yuan H
Biomolecules & biomedicine 2023 Sep 4;23(5):825-837

Chronic exposure to low-dose deltamethrin can lead to colon tissue injury through PRDX1 inactivation-induced mitochondrial oxidative stress injury and gut microbial dysbiosis.

Ma R, Sun T, Wang X, Ren K, Min T, Xie X, Wang D, Li K, Zhang Y, Zhu K, Mo C, Dang C, Yang Y, Zhang H
Ecotoxicology and environmental safety 2023 Oct 1;264:115475

Effect of peroxiredoxin 1 on the regulation of trophoblast function by affecting autophagy and oxidative stress in preeclampsia.

Zhou M, Guo J, Li S, Li A, Fang Z, Zhao M, Zhang M, Wang X
Journal of assisted reproduction and genetics 2023 Jul;40(7):1573-1587

Conoidin A, a Covalent Inhibitor of Peroxiredoxin 2, Reduces Growth of Glioblastoma Cells by Triggering ROS Production.

Szeliga M, Rola R
Cells 2023 Jul 26;12(15)

Periplocin exerts antitumor activity by regulating Nrf2-mediated signaling pathway in gemcitabine-resistant pancreatic cancer cells.

Bae ES, Byun WS, Ock CW, Kim WK, Park HJ, Lee SK
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Jan;157:114039

EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion.

Zhang ZJ, Sun ZX, Liu HJ
Heliyon 2023 Apr;9(4):e15305

Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells.

Li J, Sun Y, Zhao X, Ma Y, Xie Y, Liu S, Hui B, Shi X, Sun X, Zhang X
Cell death & disease 2023 Apr 8;14(4):259

Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control.

Wu M, Zhao A, Yan X, Gao H, Zhang C, Liu X, Luo Q, Xie F, Liu S, Shi D
eLife 2022 Sep 26;11

Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.

Prutton KM, Marentette JO, Leifheit BA, Esquer H, LaBarbera DV, Anderson CC, Maclean KN, Roede JR
Free radical biology & medicine 2022 Jun;186:32-42

Selenium Status in Diet Affects Nephrotoxicity Induced by Cisplatin in Mice.

Liu S, Wen X, Huang Q, Zhu M, Lu J
Antioxidants (Basel, Switzerland) 2022 Jun 10;11(6)

Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.

Li JW, Wang RL, Xu J, Sun KY, Jiang HM, Sun ZY, Lv ZY, Xu XQ, Wu R, Guo H, Jiang Q, Shi DQ
Acta pharmacologica Sinica 2022 Feb;43(2):417-428

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Huang H, Zhang S, Li Y, Liu Z, Mi L, Cai Y, Wang X, Chen L, Ran H, Xiao D, Li F, Wu J, Li T, Han Q, Chen L, Pan X, Li H, Li T, He K, Li A, Zhang X, Zhou T, Xia Q, Man J
Nature communications 2021 Jun 17;12(1):3720

Selenium Status in Diet Affects Acetaminophen-Induced Hepatotoxicity via Interruption of Redox Environment.

Li J, Cheng P, Li S, Zhao P, Han B, Ren X, Zhong JL, Lloyd MD, Pourzand C, Holmgren A, Lu J
Antioxidants & redox signaling 2021 Jun 10;34(17):1355-1367

Maneb alters central carbon metabolism and thiol redox status in a toxicant model of Parkinson's disease.

Anderson CC, Marentette JO, Rauniyar AK, Prutton KM, Khatri M, Matheson C, Reisz JA, Reigan P, D'Alessandro A, Roede JR
Free radical biology & medicine 2021 Jan;162:65-76

Mitochondria-targeted paraquat and metformin mediate ROS production to induce multiple pathways of retrograde signaling: A dose-dependent phenomenon.

Chowdhury AR, Zielonka J, Kalyanaraman B, Hartley RC, Murphy MP, Avadhani NG
Redox biology 2020 Sep;36:101606

Short hairpin RNA attenuates liver fibrosis by regulating the PPAR‑γ and NF‑κB pathways in HBV‑induced liver fibrosis in mice.

Ye L, Chen T, Cao J, Sun L, Li W, Zhang C
International journal of oncology 2020 Nov;57(5):1116-1128

Peroxiredoxins are involved in the pathogenesis of multiple sclerosis and neuromyelitis optica spectrum disorder.

Uzawa A, Mori M, Masuda H, Ohtani R, Uchida T, Aoki R, Kuwabara S
Clinical and experimental immunology 2020 Nov;202(2):239-248

Direct Proteomic Mapping of Cysteine Persulfidation.

Fu L, Liu K, He J, Tian C, Yu X, Yang J
Antioxidants & redox signaling 2020 Nov 20;33(15):1061-1076

MS-275 combined with cisplatin exerts synergistic antitumor effects in human esophageal squamous cell carcinoma cells.

Liu T, Guan F, Wang Y, Zhang Z, Li Y, Cui Y, Li Z, Liu H, Zhang Y, Wang Y, Ma S
Toxicology and applied pharmacology 2020 May 15;395:114971

β-Elemene enhances radiosensitivity in non-small-cell lung cancer by inhibiting epithelial-mesenchymal transition and cancer stem cell traits via Prx-1/NF-kB/iNOS signaling pathway.

Zou K, Li Z, Zhang Y, Mu L, Chen M, Wang R, Deng W, Zou L, Liu J
Aging 2020 Dec 9;13(2):2575-2592

Cyclovirobuxine D protects against diabetic cardiomyopathy by activating Nrf2-mediated antioxidant responses.

Jiang Z, Fu L, Xu Y, Hu X, Yang H, Zhang Y, Luo H, Gan S, Tao L, Liang G, Shen X
Scientific reports 2020 Apr 14;10(1):6427

The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer.

Qin W, Tian Y, Zhang J, Liu W, Zhou Q, Hu S, Yang F, Lu L, Lu H, Cui S, Wen L, Wei S
Scientific reports 2019 Sep 10;9(1):12973

MicroRNA-596 acts as a tumor suppressor in gastric cancer and is upregulated by promotor demethylation.

Zhang Z, Dai DQ
World journal of gastroenterology 2019 Mar 14;25(10):1224-1237

Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation.

Chen JL, Hung CT, Keller JJ, Lin HC, Wu YJ
BMC ophthalmology 2019 Aug 2;19(1):168

Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.

Zhu H, Wang B, Kong L, An T, Li G, Zhou H, Gong L, Zhao Z, Gong Y, Sun H, Puno P, Li Y
Cell chemical biology 2019 Aug 15;26(8):1122-1132.e6

Luteolin ameliorates rat myocardial ischaemia-reperfusion injury through activation of peroxiredoxin II.

Wei B, Lin Q, Ji YG, Zhao YC, Ding LN, Zhou WJ, Zhang LH, Gao CY, Zhao W
British journal of pharmacology 2018 Aug;175(16):3315-3332

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Jian W, Wei X, Chen L, Wang Z, Sun Y, Zhu S, Lou H, Yan S, Li X, Zhou J, Zhang B
Neuroscience letters 2017 Sep 29;658:114-120

Nucleophosmin Regulates Intracellular Oxidative Stress Homeostasis via Antioxidant PRDX6.

Liu GY, Shi JX, Shi SL, Liu F, Rui G, Li X, Gao LB, Deng XL, Li QF
Journal of cellular biochemistry 2017 Dec;118(12):4697-4707

The role of peroxiredoxin I in cisplatin-induced ototoxicity.

Le Q, Tabuchi K, Warabi E, Hara A
Auris, nasus, larynx 2017 Apr;44(2):205-212

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Dong K, Wu M, Liu X, Huang Y, Zhang D, Wang Y, Yan LJ, Shi D
Free radical biology & medicine 2016 Dec;101:334-347

Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1.

Liu L, Ma Y, Zhou H, Wu M
International journal of molecular sciences 2016 Aug 27;17(9)

Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.

Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ
Journal of agricultural and food chemistry 2014 Dec 3;62(48):11672-80

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Wu H, Xiao Y, Zhang S, Ji S, Wei L, Fan F, Geng J, Tian J, Sun X, Qin F, Jin C, Lin J, Yin ZY, Zhang T, Luo L, Li Y, Song S, Lin SC, Deng X, Camargo F, Avruch J, Chen L, Zhou D
Cell reports 2013 May 30;3(5):1663-77

Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.

Zhao X, Cong X, Zheng L, Xu L, Yin L, Peng J
Toxicology letters 2012 Oct 2;214(1):69-80

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Liu C, Zhang A, Guo J, Yang J, Zhou H, Chen H, Jin M
Journal of proteome research 2012 Nov 2;11(11):5396-405

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 15816-1-AP(PRDX1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PRDX1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PRDX1. This antibody recognizes human, mouse, rat antigen. The PRDX1 antibody has been validated for the following applications: ELISA, WB, IF, IHC, IP analysis.
Appendix: 15816-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-PRDX1 (IP:15816-1-AP, 3ug; Detection:15816-1-AP 1:1000) with HeLa cells lysate 2400ug.
Sample type: cell line