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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- R31761 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRDX1 Antibody (Peroxiredoxin 1)
- Antibody type
- Polyclonal
- Description
- This highly specific PRDX1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- The PRDX1 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of PRDX1 antibody and Lane 1: rat brain; 2: mouse brain; 3: human U87; 4: (m) Neuro-2a; 5: (h) A375; 6: (h) 293T; 7: (h) SMMC; 8: (h) A549; 9: (h) RH35 lysate. Expected size: ~22KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat kidney, 2) rat testis, 3) rat liver, 4) rat PC-12, 5) mouse kidney, 6) mouse testis, 7) mouse liver and 8) mouse RAW264.7 cell lysate with PRDX1 antibody. Expected molecular weight: ~22 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) Raji, 2) Jurkat, 3) MDA-MB-453, 4) HeLa, 5) HepG2, 6) HEK293, 7) human placenta and 8) A549 cell lysate with PRDX1 antibody. Expected molecular weight: ~22 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of PRDX1 antibody and (h) SMMC-7721 cells
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with PRDX1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of PRDX1 antibody and human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
