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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab121313 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Peroxiredoxin 1/PAG Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Peroxiredoxin 1/PAG Antibody, Recombinant, could be used for WB, ICC, IF and so on.ApplicationWB: 1/500-1/1000ICC/IF: 1/50-1/200Protein Function Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 186-199- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,2 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Peroxiredoxin 1/PAG Antibody (Ab121313) - Western Blot All lanes: Recombinant Peroxiredoxin 1/PAG Antibody (Ab121313) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 24 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Peroxiredoxin 1/PAG Antibody (Ab121313) - IF/ICC Immunocytochemistry analysis of Peroxiredoxin 1 (green) in HeLa using Recombinant Peroxiredoxin 1/PAG Antibody (Ab121313), and cells were counterstained with DAPI (blue).
