GTX102996

antibody from GeneTex

Targeting: COL1A2 OI4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX102996

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102996, RRID:AB_1950011

Product name

COL1A2 antibody [C2C3], C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Kaempferol inhibits fibroblast collagen synthesis, proliferation and activation in hypertrophic scar via targeting TGF-β receptor type I.

Li H, Yang L, Zhang Y, Gao Z
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2016 Oct;83:967-974

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COL1A2 antibody [C2C3], C-term detects COL1A2 protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g whole cell extract of GFP-human COL1A2-transfected 293T cells (partial)10 % SDS-PAGECOL1A2 antibody [C2C3], C-term (GTX102996) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COL1A2 antibody [C2C3], C-term detects COL1A2 protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g whole cell extract of GFP-human COL1A2-transfected 293T cells (partial)10% SDS-PAGECOL1A2 antibody [C2C3], C-term (GTX102996) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

COL1A2 antibody [C2C3], C-term detects COL1A2 protein at cytoplasm by immunofluorescent analysis.Sample: NIH-3T3 cells were fixed in ice-cold MeOH for 5 min.Green: COL1A2 protein stained by COL1A2 antibody [C2C3], C-term (GTX102996) diluted at 1:500.Blue: Hoechst 33342 staining.