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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- RQ5202 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Collagen I Antibody / COL1A1
- Antibody type
- Monoclonal
- Description
- This highly specific Collagen I antibody is suitable for use in Immunohistochemistry/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- AFD-3
- Vial size
- 100 ul
- Concentration
- Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
- Storage
- Store the Collagen I antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human stomach lysate with Collagen I antibody. Expected molecular weight: 100-140 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human stomach lysate with Collagen I antibody. Expected molecular weight: 100-140 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney tissue with Collagen I antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung squamous cell carcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 10-20 min and allow to cool before testing.
