Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total SLC16A7 / MCT2 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC16A7 was performed by loading A549 cell lysates (30µg) on to a 10+12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a SLC16A7 antibody (Product # PA5-50518) at a 1/1200 dilution for 40 seconds, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MCT2 Polyclonal Antibody (Product # PA5-50518) and a 48 kDa band corresponding to Monocarboxylate transporter 2 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), MOLT-4 (Lane 4), THP-1 (Lane 5), Jurkat (Lane 6), BeWo (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MCT2 in paraffin embedded Human ovarian cancer tissue using MCT2 Polyclonal Antibody (Product # PA5-50518) at a 1:70 dilution.