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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-52225 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GPNQTTSKSK NKTGKTEDDS SPKKIKTKKS TWEKVNKYLD FS
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCT2 in control (vector only transfected HEK293T lysate) and SLC16A7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MCT2 Polyclonal Antibody (Product # PA5-52225).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MCT2 Polyclonal Antibody (Product # PA5-52225) and a 48 kDa band corresponding to Monocarboxylate transporter 2 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2), U-87 MG (Lane 3), MOLT-4 (Lane 4), THP-1 (Lane 5), Jurkat (Lane 6), BeWo (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MCT2 in human cerebral cortex tissue shows moderate cytoplasmic positivity in neuronal cells. Samples were probed using a MCT2 Polyclonal Antibody (Product # PA5-52225).
