TA800429

antibody from OriGene

Targeting: ACADS ACAD3, SCAD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800429

Provider

OriGene

Proper citation

OriGene Cat#TA800429, RRID:AB_2625440

Product name

ACADS mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Antibody type

Monoclonal

Description

ACADS mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Host

Mouse

Conjugate

Unconjugated

Epitope

ACADS

Isotype

IgG

Antibody clone number

OTI1D2

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACADS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACADS (RC206855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACADS.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACADS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800429)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACADS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800429)

Validation comment

IHC