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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-54580 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACADS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ELPETHQMLL QTCRDFAEKE LFPIAAQVDK EHLFPAAQVK KMGGLGLLAM DVPEELGGAG LDYLAYAIAM EEISRGCAST GVIMSVNNSL YLGPILKFGS KEQKQAWVTP FTSGDKIGCF ALSEPGNGS Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation.
Hao YH, Lafita-Navarro MC, Zacharias L, Borenstein-Auerbach N, Kim M, Barnes S, Kim J, Shay J, DeBerardinis RJ, Conacci-Sorrell M
Cell communication and signaling : CCS 2019 Oct 17;17(1):129
Cell communication and signaling : CCS 2019 Oct 17;17(1):129
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACADS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACADS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ACADS in human cell line U-251 MG shows positivity in cytoplasm, centrosome & nucleus but excluded from the nucleoli. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ACADS in human cell line Hep G2 using ACADS Polyclonal Antibody (Product # PA5-54580) shows localization to mitochondria.
