PA5-54580

antibody from Invitrogen Antibodies

Targeting: ACADS ACAD3, SCAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54580

Provider

Invitrogen Antibodies

Product name

ACADS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ELPETHQMLL QTCRDFAEKE LFPIAAQVDK EHLFPAAQVK KMGGLGLLAM DVPEELGGAG LDYLAYAIAM EEISRGCAST GVIMSVNNSL YLGPILKFGS KEQKQAWVTP FTSGDKIGCF ALSEPGNGS

Concentration

0.3 mg/mL

References

Submitted references

Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation.

Hao YH, Lafita-Navarro MC, Zacharias L, Borenstein-Auerbach N, Kim M, Barnes S, Kim J, Shay J, DeBerardinis RJ, Conacci-Sorrell M
Cell communication and signaling : CCS 2019 Oct 17;17(1):129

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACADS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACADS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ACADS in human cell line U-251 MG shows positivity in cytoplasm, centrosome & nucleus but excluded from the nucleoli. Samples were probed using an ACADS Polyclonal Antibody (Product # PA5-54580).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ACADS in human cell line Hep G2 using ACADS Polyclonal Antibody (Product # PA5-54580) shows localization to mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human liver using an ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity with a granular pattern in hepatocytes and bile duct cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human duodenum using ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human liver using ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human kidney using ACADS Polyclonal Antibody (Product # PA5-54580) shows strong cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human testis using ACADS Polyclonal Antibody (Product # PA5-54580) shows moderate cytoplasmic positivity in Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ACADS in human tonsil using ACADS Polyclonal Antibody (Product # PA5-54580) shows granular cytoplasmic positivity in germinal center cells.