PA5-78544

antibody from Invitrogen Antibodies

Targeting: SLC12A5 KCC2, KIAA1176

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-78544 - Provider product page
Provider: Invitrogen Antibodies
Product name: KCC2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Control: SK-N-SH, mouse hippocampus, rat hippocampus, rat spinal cord Predicted Reactivity: Mouse (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.36 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SLC12A5 protein structure - PA5-78544 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KCC2 in rat tissue extract using 50 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with KCC2 polyclonal antibody (Product # PA5-78544) using a dilution of 1:5000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KCC2 in mouse tissue extract using 50 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with KCC2 polyclonal antibody (Product # PA5-78544) using a dilution of 1:5000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KCC2 in rat tissue extract using 50 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with KCC2 polyclonal antibody (Product # PA5-78544) using a dilution of 1:5000 followed by HRP-conjugated anti-rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KCC2 in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with KCC2 polyclonal antibody (Product # PA5-78544) using a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using KCC2 Polyclonal Antibody (Product # PA5-78544). Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 Polyclonal Antibody (Product # PA5-78544) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using KCC2 Polyclonal Antibody (Product # PA5-78544). Rat tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 Polyclonal Antibody (Product # PA5-78544) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using KCC2 Polyclonal Antibody (Product # PA5-78544). Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 Polyclonal Antibody (Product # PA5-78544) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using KCC2 Polyclonal Antibody (Product # PA5-78544). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 Polyclonal Antibody (Product # PA5-78544) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-KCC2 Polyclonal Antibody (Product # PA5-78544) and ~126 kDa band corresponding to KCC2 was observed in Mouse Brain and Rat Brain, but not in Mouse Stomach and Rat Stomach which are reported to be negative. An uncharacterized band at ~45 kDa, 55 kDa were observed in the brain tissues tested. Tissue lysate (40ug lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Stomach (Lane 3), and Rat Stomach (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).