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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-27610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCC2 Monoclonal Antibody (N1/12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27610 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa. This antibody was formerly sold as clone S1-12
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N1/12
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references TMEM16A expression in cholinergic neurons of the medial habenula mediates anxiety-related behaviors.
Cho CH, Lee S, Kim A, Yarishkin O, Ryoo K, Lee YS, Jung HG, Yang E, Lee DY, Lee B, Kim H, Oh U, Im HI, Hwang EM, Park JY
EMBO reports 2020 Feb 5;21(2):e48097
EMBO reports 2020 Feb 5;21(2):e48097
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KCC2 in rat brain membrane lysate. The sample was incubated with KCC2 monoclonal antibody (Product # MA5-27610) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-KCC2 Polyclonal Antibody (Product # MA5-27610) and ~126 kDa band corresponding to KCC2 was observed in Mouse Brain and Rat Brain, but not in Mouse Stomach and Rat Stomach as reported. Tissue lysate (40ug lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Stomach (Lane 3), and Rat Stomach (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KCC2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KCC2 monoclonal antibody (Product # MA5-27610) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) KCC2 Antibody, D) Merged image. Magnification: 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
