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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005459-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005459-M01, RRID:AB_547210
- Product name
- Mouse Monoclonal POU4F3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. POU4F3 - POU domain, class 4, transcription factor 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi ( Cat # H00005459-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 (Cat # H00005459-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - POU4F3 monoclonal antibody (M01), clone 5B8 Analysis of POU4F3 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: POU4F3 Antibody (5B8) [H00005459-M01] - Detection limit for recombinant GST tagged POU4F3 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of monoclonal antibody to POU4F3 on HeLa cell. Antibody concentration 10 ug/ml.
