Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B11296 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ TCERG1 / CA150 Antibody (aa1-50) LS-B11296
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human CA150 by Western Blot. Sample: Nuclear extract (5 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-CA150 used at 0.33 ug/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3000 with NBT-BCIP as substrate for 20 min.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human CA150 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CA150 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.