Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-60554 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60554, RRID:AB_922242
- Product name
- Goat Polyclonal Perilipin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Mosaic Mutant Analysis Identifies PDGFRα/PDGFRβ as Negative Regulators of Adipogenesis.
Partial leptin deficiency confers resistance to diet-induced obesity in mice.
Peroxisome Proliferator-Activated Receptor γ and Its Role in Adipocyte Homeostasis and Thiazolidinedione-Mediated Insulin Sensitization.
Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo.
PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis.
Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation.
Rapid cellular turnover in adipose tissue.
Perilipin 1 and perilipin 2 protein localization and gene expression study in skeletal muscles of European cross-breed pigs with different intramuscular fat contents.
A proposed model of fat packaging by exchangeable lipid droplet proteins.
Sun C, Sakashita H, Kim J, Tang Z, Upchurch GM, Yao L, Berry WL, Griffin TM, Olson LE
Cell stem cell 2020 May 7;26(5):707-721.e5
Cell stem cell 2020 May 7;26(5):707-721.e5
Partial leptin deficiency confers resistance to diet-induced obesity in mice.
Zhao S, Li N, Zhu Y, Straub L, Zhang Z, Wang MY, Zhu Q, Kusminski CM, Elmquist JK, Scherer PE
Molecular metabolism 2020 Jul;37:100995
Molecular metabolism 2020 Jul;37:100995
Peroxisome Proliferator-Activated Receptor γ and Its Role in Adipocyte Homeostasis and Thiazolidinedione-Mediated Insulin Sensitization.
Wang QA, Zhang F, Jiang L, Ye R, An Y, Shao M, Tao C, Gupta RK, Scherer PE
Molecular and cellular biology 2018 May 15;38(10)
Molecular and cellular biology 2018 May 15;38(10)
Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo.
Nakazeki F, Nishiga M, Horie T, Nishi H, Nakashima Y, Baba O, Kuwabara Y, Nishino T, Nakao T, Ide Y, Koyama S, Kimura M, Tsuji S, Sowa N, Yoshida S, Conway SJ, Yanagita M, Kimura T, Ono K
Scientific reports 2018 Jun 4;8(1):8553
Scientific reports 2018 Jun 4;8(1):8553
PDGFRα controls the balance of stromal and adipogenic cells during adipose tissue organogenesis.
Sun C, Berry WL, Olson LE
Development (Cambridge, England) 2017 Jan 1;144(1):83-94
Development (Cambridge, England) 2017 Jan 1;144(1):83-94
Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation.
Wang QA, Tao C, Jiang L, Shao M, Ye R, Zhu Y, Gordillo R, Ali A, Lian Y, Holland WL, Gupta RK, Scherer PE
Nature cell biology 2015 Sep;17(9):1099-111
Nature cell biology 2015 Sep;17(9):1099-111
Rapid cellular turnover in adipose tissue.
Rigamonti A, Brennand K, Lau F, Cowan CA
PloS one 2011 Mar 2;6(3):e17637
PloS one 2011 Mar 2;6(3):e17637
Perilipin 1 and perilipin 2 protein localization and gene expression study in skeletal muscles of European cross-breed pigs with different intramuscular fat contents.
Gandolfi G, Mazzoni M, Zambonelli P, Lalatta-Costerbosa G, Tronca A, Russo V, Davoli R
Meat science 2011 Aug;88(4):631-7
Meat science 2011 Aug;88(4):631-7
A proposed model of fat packaging by exchangeable lipid droplet proteins.
Wolins NE, Brasaemle DL, Bickel PE
FEBS letters 2006 Oct 9;580(23):5484-91
FEBS letters 2006 Oct 9;580(23):5484-91
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Perilipin Antibody [NB100-60554] - (0.01ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Perilipin Antibody [NB100-60554] - Staining of Human Adipose lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Perilipin Antibody [NB100-60554] - (4 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Perilipin Antibody [NB100-60554] - Staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USA.
