PA5-55046

antibody from Invitrogen Antibodies

Targeting: PLIN1 PLIN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55046

Provider

Invitrogen Antibodies

Product name

Perilipin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VCNAYEKGVQ SASSLAAWSM EPVVRRLSTQ FTAANELACR GLDHLEEKIP ALQYPPEKIA SELKDTISTR LRSARNSISV PIASTSDKVL GAALAGCELA WGVARDTAEF AANTRAGRLA SGGADLALGS IEKVVE

Concentration

0.08 mg/mL

References

Submitted references

Xenogen-free isolation and culture of human adipose mesenchymal stem cells.

Doornaert M, De Maere E, Colle J, Declercq H, Taminau J, Lemeire K, Berx G, Blondeel P
Stem cell research 2019 Oct;40:101532

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Perilipin 1 Polyclonal Antibody (Product # PA5-55046) and a 60kDa band corresponding to Perilipin 1 was observed across along with uncharacterized band (*). Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2) as seen in Fig (a). Whole cell extracts (30 µg lysate) of FaDu (Lane 1), HCT 116 (Lane 2), SiHa (Lane 3), BeWo (Lane 4), Hep G2 (Lane 5) and IMR-32 (Lane 6) as seen in Fig (b). Tissue Extracts of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Testis (Lane 3), Rat Testis (Lane 4), Mouse Skeletal Muscle (Lane 5), Mouse Adipose (Lane 6), Mouse Brown Adipose (Lane 7), Rat Adipose (Lane 8) and Rat Brown Adipose (Lane 9) as seen in Fig (c), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Proteins were then transferred onto a membrane (Product # IB23002) by iBlot® 2 System (Product # IB21001). The blot was probed with primary antibody (1:200 dilution) and detected with Goat anti-Rabbit IgG (H+L) Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Detection was performed using Novex® ECL Substrate Reagent Kit (Product # WP20005). Perilipin 1 was observed to be high in Mouse Brown Adipose, Mouse and Rat White adipose and Rat Brown adipose in comparison to Low or negative in Mouse testis ,Rat Testis, Mouse Kidney, Rat Kidney and Mouse Skeletal Muscle, an induction in protein expression.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Perilipin 1 was performed using 100% confluent log phase differentiated 3T3-L1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perilipin 1 Polyclonal Antibody (Product # PA5-55046) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d and e represent the merged image showing protein localized to lipid droplets, with different stages of adipocyte differentiation. Panel f represents undifferentiated 3T3-L1 cells. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Perilipin in human breast tissue shows strong positivity in adipocytes. Samples were probed using a Perilipin Polyclonal Antibody (Product # PA5-55046).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Perilipin 1 in human breast and placenta tissues using Perilipin 1 Polyclonal Antibody (Product # PA5-55046). Corresponding PLIN1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Perilipin 1 in human placenta using Perilipin 1 Polyclonal Antibody (Product # PA5-55046) shows low expression as expected.