PA5-18694

antibody from Invitrogen Antibodies

Targeting: PLIN1 PLIN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18694

Provider

Invitrogen Antibodies

Product name

Perilipin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human Adipose lysate using Product # PA5-18694 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Perilipin 1 Polyclonal Antibody (Product # PA5-18694) and a 60 kDa band corresponding to Perilipin-1 was observed in 3T3-L1 differentiated Adipocytes and Mouse Adipose tissue but not in 3T3-L1 cells or Mouse Kidney. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated into Adipocytes (Lane 2), tissue lysate of Mouse Adipose tissue (Lane 3) and Mouse Kidney (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).