Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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Validation data
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- Product number
- NB110-40760 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40760, RRID:AB_715043
- Product name
- Rabbit Polyclonal Perilipin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Evaporative cooling provides a major metabolic energy sink.
Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis.
Human adipose beiging in response to cold and mirabegron.
PCB and OCP accumulation and evidence of hepatic alteration in the Atlantic bluefin tuna, T. thynnus, from the Mediterranean Sea.
Evolutionarily conserved gene family important for fat storage.
Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.
Kasza I, Adler D, Nelson DW, Eric Yen CL, Dumas S, Ntambi JM, MacDougald OA, Hernando D, Porter WP, Best FA, Alexander CM
Molecular metabolism 2019 Sep;27:47-61
Molecular metabolism 2019 Sep;27:47-61
Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis.
Listenberger L, Townsend E, Rickertsen C, Hains A, Brown E, Inwards EG, Stoeckman AK, Matis MP, Sampathkumar RS, Osna NA, Kharbanda KK
Cells 2018 Nov 24;7(12)
Cells 2018 Nov 24;7(12)
Human adipose beiging in response to cold and mirabegron.
Finlin BS, Memetimin H, Confides AL, Kasza I, Zhu B, Vekaria HJ, Harfmann B, Jones KA, Johnson ZR, Westgate PM, Alexander CM, Sullivan PG, Dupont-Versteegden EE, Kern PA
JCI insight 2018 Aug 9;3(15)
JCI insight 2018 Aug 9;3(15)
PCB and OCP accumulation and evidence of hepatic alteration in the Atlantic bluefin tuna, T. thynnus, from the Mediterranean Sea.
Maisano M, Cappello T, Oliva S, Natalotto A, Giannetto A, Parrino V, Battaglia P, Romeo T, Salvo A, Spanò N, Mauceri A
Marine environmental research 2016 Oct;121:40-8
Marine environmental research 2016 Oct;121:40-8
Evolutionarily conserved gene family important for fat storage.
Kadereit B, Kumar P, Wang WJ, Miranda D, Snapp EL, Severina N, Torregroza I, Evans T, Silver DL
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):94-9
Proceedings of the National Academy of Sciences of the United States of America 2008 Jan 8;105(1):94-9
Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.
Manzano-Roman R, Almazán C, Naranjo V, Bloui EF, Kocan KM, de la Fuente J
Journal of medical microbiology 2008 Feb;57(Pt 2):159-163
Journal of medical microbiology 2008 Feb;57(Pt 2):159-163
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Perilipin Antibody [NB110-40760] - Total protein from Human breast (lane 1), Human adipose membrane fraction (lane 2) and Human adipose tissue (lane 3) were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, probed with 2 ug/ml anti-perilipin antibody followed by anti-rabbit HRP conjugated secondary antibody and detected with chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of liver tissue from mouse with Perilipin antibody at 1:200 dilution. The hepatocytes developed specific staining in the cytoplasm and around the nuclei of some cells, the immunostaining reflected a punctate appearance which is potentially the ER of the cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Perilipin Antibody [NB110-40760] - IHC analysis of formalin fixed paraffin embedded section of fat tissue from mouse with Perilipin antibody at 1:200 dilution. The antibody generated an expected staining in the adipocytes towards periphery of the cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on MCF7 cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Perilipin Antibody [NB110-40760] - An intracellular stain was performed on U2OS cells with Perilipin Antibody NB110-40760AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.