HPA004122

antibody from Atlas Antibodies

Targeting: CTCF CFAP108, FAP108

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA004122

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004122, RRID:AB_2667139

Product name

Anti-CTCF

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human CTCF, Gene description: CCCTC-binding factor (zinc finger protein), Validated applications: IHC, ICC, ChIP, Uniprot ID: P49711, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.05 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Divergent transcriptional regulation of astrocyte reactivity across disorders

Burda J, O’Shea T, Ao Y, Suresh K, Wang S, Bernstein A, Chandra A, Deverasetty S, Kawaguchi R, Kim J, McCallum S, Rogers A, Wahane S, Sofroniew M
Nature 2022;606(7914):557-564

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Expression of CCCTC‐binding factor (CTCF) is linked to poor prognosis in prostate cancer

Höflmayer D, Steinhoff A, Hube‐Magg C, Kluth M, Simon R, Burandt E, Tsourlakis M, Minner S, Sauter G, Büscheck F, Wilczak W, Steurer S, Huland H, Graefen M, Haese A, Heinzer H, Schlomm T, Jacobsen F, Hinsch A, Poos A, Oswald M, Rippe K, König R, Schroeder C
Molecular Oncology 2019;14(1):129-138

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-CTCF (HPA004122, Lot A96611) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.