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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [6]
- Immunohistochemistry [4]
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- Product number
- LS-C331282 - Provider product page
- Provider
- LSBio
- Product name
- CTCF Antibody LS-C331282
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis extracts of HCT116 cells, using CTCF antibodyand rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse heart tissue.
