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- Product number
- PA5-46847 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTCF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE
- Concentration
- 0.5 mg/mL
Submitted references Natural Antisense Transcript PEBP1P3 Regulates the RNA Expression, DNA Methylation and Histone Modification of CD45 Gene.
Su Z, Liu G, Zhang B, Lin Z, Huang D
Genes 2021 May 17;12(5)
Genes 2021 May 17;12(5)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of human 293T cells using an anti-CTCF polyclonal antibody (Product # PA5-46847).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay analysis of HCT116 cells using an anti-CTCF polyclonal antibody (Product # PA5-46847).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 The effect of PEBP1P3 knockdown on DNA and histone modification of CD45. ( A , B ) The DNA methylation status and methylation ratio of CpG sites on CD45 intron 2 (I2) and exon 5 (E5) after PEBP1P3 was knocked down in HuT 78, Jurkat and RPMI 8226 cells. Closed circles represent methylated CpG sites, and open circles represent unmethylated CpG sites. ( C ) The sketch indicates the locational relationship of the CD45 and PEBP1P3 genes, CD45 RNA isoforms, locations of primers applied in ChIP and two potential CTCF binding sites predicted through online CTCFBSDB 2.0. ( D ) The levels of trimethylated histone H3K4 (H3K4me3), trimethylated histone H3K9 (H3K9me3), trimethylated histone H3K36 (H3K36me3), acetylated histone (H3acetyl), acetylated H3K9 (H3K9ac) and CTCF binding at different locations of CD45 DNA after PEBP1P3 was knocked down in HuT 78 cells. Each experiment was repeated three times, and the quantification was presented as the mean +- SEM. si-NC: interference of negative control; si-PEBP1P3, interference of PEBP1P3; * p < 0.05.
