LS-C745341

antibody from LSBio

Targeting: CTCF CFAP108, FAP108

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745341

Provider

LSBio

Product name

CTCF Antibody (C-Terminus) LS-C745341

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of rabbit Anti-CTCF antibody. Lane 1: P3XAg nuclear lysate. Lane 2: Flag-CTCF. Load: 30 µg per lane. Primary antibody: CTCF antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 µg per lane. Primary antibody: CTCF antibody at 1.2 µg/mL for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 3% BSA overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of rabbit anti-CTCF antibody. Tissue: tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-CTCF at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: CTCF as precipitated red signal with hematoxylin purple nuclear counterstain.