PA5-88430

antibody from Invitrogen Antibodies

Targeting: SELENBP1 hSBP, hSP56, LPSB

Antibody data

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Validation data

Product number: PA5-88430 - Provider product page
Provider: Invitrogen Antibodies
Product name: SELENBP1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: SLKDGLIPLE IRFLHNPDAA QGFVGCALSS TIQRFYKNEG GTWSVEKVIQ VPPKKVKGWL LPEMPGLITD ILLSLDDRFL YFSNWLHGDL RQYDISDPQR PRLTGQLFLG GSIVKGGPVQ VLEDEELKSQ PEPLVVKGKR VAGGPQMIQL SLDGKRLYIT TSLYSAWDKQ FYPDLIREGS VMLQVDVDTV KGGLKLNPNF LVDFGKEPLG PALAHELRYP GGDCSSDIWI; Positive Samples: MCF7, Mouse liver, Mouse kideny; Cellular Location: Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytosol
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.96 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

SELENBP1 protein structure - PA5-88430 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using SELENBP1 Polyclonal antibody (Product # PA5-88430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 Polyclonal antibody (Product # PA5-88430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SELENBP1 in extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells using SELENBP1 Polyclonal Antibody (Product # PA5-88430) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SELENBP1 in extracts of various cell lines using SELENBP1 Polyclonal Antibody (Product # PA5-88430) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.