Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-21079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DPF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse kidney tissue lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse kidney tissue lysate using a REQUIEM polyclonal antibody (Product # PA5-21079) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Requiem in mouse kidney tissue lysate with DPF2 Polyclonal Antibody (Product # PA5-21079) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DPF2 Polyclonal Antibody (Product # PA5-21079) and a 45 kDa band corresponding to Zinc finger protein ubi-d4 was observed along with uncharacterized bands (*) at 160 kDa across cell lines and tissue tested. Nuclear enriched extracts (30 µg lysate) of A-431 (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3) and tissue extract of Mouse Thymus (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Zinc finger protein ubi-d4 was achieved by transfecting K-562 with Zinc finger protein ubi-d4 specific siRNAs (Silencer® select Product # S11929, S11930). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Zinc finger protein ubi-d4 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with DPF2 Polyclonal Antibody (Product # PA5-21079, 1 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Zinc finger protein ubi-d4.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of REQUIEM in human kidney tissue with DPF2 Polyclonal Antibody (Product # PA5-21079) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of REQUIEM in human kidney tissue with DPF2 Polyclonal Antibody (Product # PA5-21079) at 5 µg/mL.