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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-63886 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIMELESS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FVELLFWKNT AVVREMTEGY GSLDDRSSSR RAPTWSPEEE AHLQELYLAN KDVEGQDVVE AILAHLNTVP RTRKQIIHHL VQMGLADSVK DFQR
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TIMELESS in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line U-251 MG. Samples were probed using a TIMELESS Polyclonal Antibody (Product # PA5-63886).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TIMELESS in human cell line MCF7 using a TIMELESS Polyclonal Antibody (Product # PA5-63886) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TIMELESS in human testis tissue shows strong nuclear positivity in subset of cells in seminiferus ducts. Samples were probed using a TIMELESS Polyclonal Antibody (Product # PA5-63886).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TIMELESS in human testis and skeletal muscle tissues using TIMELESS Polyclonal Antibody (Product # PA5-63886). Corresponding TIMELESS RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TIMELESS in human skeletal muscle using TIMELESS Polyclonal Antibody (Product # PA5-63886) shows low expression as expected.
