PA5-78270

antibody from Invitrogen Antibodies

Targeting: TIMELESS hTIM, TIM, TIM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78270

Provider

Invitrogen Antibodies

Product name

TIMELESS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TIMELESS in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with TIMELESS polyclonal antibody (Product # PA5-78270) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TIMELESS Polyclonal Antibody detects TIMELESS protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. TIMELESS Polyclonal Antibody (Product # PA5-78270) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TIMELESS Polyclonal Antibody detects TIMELESS protein by western blot analysis. A. 30 µg Neuro2A whole cell extract. B. 30 µg C8D30 whole cell extract. C. 30 µg NIH-3T3 whole cell extract. D. 30 µg Raw 264.7 whole cell extract. E. 30 µg C2Cl2 whole cell extract.5 % SDS-PAGE. TIMELESS Polyclonal Antibody (Product # PA5-78270) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TIMELESS Polyclonal Antibody detects TIMELESS protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TIMELESS protein stained by TIMELESS Polyclonal Antibody (Product # PA5-78270) diluted at 1:2,000. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TIMELESS in 293T whole cell extract. Samples were treated with TIMELESS polyclonal antibody (Product # PA5-78270) using a dilution of 5 µg followed by anti-rabbit IgG.