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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28026 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIMELESS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji. Predicted reactivity: Mouse (90%), Rat (90%), Sheep (93%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TIMELESS Polyclonal Antibody (Product # PA5-28026). Sample (30 µg whole cell lysate). A: HeLa S3. 7.5% SDS PAGE. TIMELESS Polyclonal Antibody (Product # PA5-28026) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TIMELESS in paraformaldehyde-fixed HeLa cells using a TIMELESS polyclonal antibody (Product # PA5-28026) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TIMELESS (Product # PA5-28026) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
