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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 15932-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15932-1-AP, RRID:AB_2264612
- Product name
- IDH2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated IDH2 antibody (Cat. #15932-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.
STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.
Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.
Two human patient mitochondrial pyruvate carrier mutations reveal distinct molecular mechanisms of dysfunction.
Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.
Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.
Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
Loss of NAD-Dependent Protein Deacetylase Sirtuin-2 Alters Mitochondrial Protein Acetylation and Dysregulates Mitophagy.
Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.
SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.
On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
MiR-181a regulates lipid metabolism via IDH1.
Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.
Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.
Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.
Pang JL, Huang FH, Zhang YH, Wu Y, Ge XM, Li S, Li X
Toxicology and applied pharmacology 2021 Nov 1;430:115726
Toxicology and applied pharmacology 2021 Nov 1;430:115726
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794
Oxidative medicine and cellular longevity 2020;2020:5849794
Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.
Chen X, Zhuo S, Xu W, Chen X, Huang D, Sun X, Cheng Y
British journal of cancer 2020 Jul;123(1):126-136
British journal of cancer 2020 Jul;123(1):126-136
STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.
Oberhuber M, Pecoraro M, Rusz M, Oberhuber G, Wieselberg M, Haslinger P, Gurnhofer E, Schlederer M, Limberger T, Lagger S, Pencik J, Kodajova P, Högler S, Stockmaier G, Grund-Gröschke S, Aberger F, Bolis M, Theurillat JP, Wiebringhaus R, Weiss T, Haitel A, Brehme M, Wadsak W, Griss J, Mohr T, Hofer A, Jäger A, Pollheimer J, Egger G, Koellensperger G, Mann M, Hantusch B, Kenner L
Molecular systems biology 2020 Apr;16(4):e9247
Molecular systems biology 2020 Apr;16(4):e9247
Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.
Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H, Maimouni S, Dai W, Velasquez S, Pan JA, Muthalagu N, Morton J, Anthony TG, Feng H, Lamers WH, Murphy DJ, Guo JY, Jin J, Crawford HC, Zhang L, White E, Lin RZ, Su X, Tuveson DA, Zong WX
Cell reports 2019 Oct 29;29(5):1287-1298.e6
Cell reports 2019 Oct 29;29(5):1287-1298.e6
Two human patient mitochondrial pyruvate carrier mutations reveal distinct molecular mechanisms of dysfunction.
Oonthonpan L, Rauckhorst AJ, Gray LR, Boutron AC, Taylor EB
JCI insight 2019 May 30;5
JCI insight 2019 May 30;5
Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.
Ju R, Fei K, Li S, Chen C, Zhu L, Li J, Zhang D, Guo L, Ye C
The Journal of pharmacology and experimental therapeutics 2018 Oct;367(1):20-27
The Journal of pharmacology and experimental therapeutics 2018 Oct;367(1):20-27
Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.
White K, Kim MJ, Han C, Park HJ, Ding D, Boyd K, Walker L, Linser P, Meneses Z, Slade C, Hirst J, Santostefano K, Terada N, Miyakawa T, Tanokura M, Salvi R, Someya S
Scientific reports 2018 Mar 22;8(1):5039
Scientific reports 2018 Mar 22;8(1):5039
Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.
Wu L, Cao KX, Ni ZH, Li WD, Chen ZP, Cheng HB, Liu X
Journal of ethnopharmacology 2018 Aug 10;222:71-78
Journal of ethnopharmacology 2018 Aug 10;222:71-78
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
Sun Y, Tian Z, Liu N, Zhang L, Gao Z, Sun X, Yu M, Wu J, Yang F, Zhao Y, Ren H, Chen H, Zhao D, Wang Y, Dong S, Xu C, Lu F, Zhang W
Journal of molecular medicine (Berlin, Germany) 2018 Apr;96(3-4):281-299
Journal of molecular medicine (Berlin, Germany) 2018 Apr;96(3-4):281-299
The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.
Chen X, Xu W, Wang C, Liu F, Guan S, Sun Y, Wang X, An D, Wen Z, Chen P, Cheng Y
American journal of cancer research 2017;7(3):700-714
American journal of cancer research 2017;7(3):700-714
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825
EMBO reports 2017 May;18(5):809-825
Loss of NAD-Dependent Protein Deacetylase Sirtuin-2 Alters Mitochondrial Protein Acetylation and Dysregulates Mitophagy.
Liu G, Park SH, Imbesi M, Nathan WJ, Zou X, Zhu Y, Jiang H, Parisiadou L, Gius D
Antioxidants & redox signaling 2017 May 20;26(15):849-863
Antioxidants & redox signaling 2017 May 20;26(15):849-863
Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.
Zang S, Tang Q, Dong F, Liu H, Li L, Guo F, Pan X, Lin H, Zeng W, Cai Z, Zhong Q, Zang N, Zang L
Oncology reports 2017 Dec;38(6):3583-3591
Oncology reports 2017 Dec;38(6):3583-3591
SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.
Zou X, Zhu Y, Park SH, Liu G, O'Brien J, Jiang H, Gius D
Cancer research 2017 Aug 1;77(15):3990-3999
Cancer research 2017 Aug 1;77(15):3990-3999
On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.
Heo H, Kim S, Lee HH, Cho HR, Xu WJ, Lee SH, Park CK, Park S, Choi SH, Kim H
PloS one 2016;11(1):e0147794
PloS one 2016;11(1):e0147794
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831
Scientific reports 2016 Mar 9;6:22831
MiR-181a regulates lipid metabolism via IDH1.
Chu B, Wu T, Miao L, Mei Y, Wu M
Scientific reports 2015 Mar 5;5:8801
Scientific reports 2015 Mar 5;5:8801
Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.
Wen H, Cho HR, Yun T, Kim H, Park CK, Lee SH, Choi SH, Park S
Journal of neurochemistry 2015 Jan;132(2):183-93
Journal of neurochemistry 2015 Jan;132(2):183-93
Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.
Ma X, Li C, Sun L, Huang D, Li T, He X, Wu G, Yang Z, Zhong X, Song L, Gao P, Zhang H
Nature communications 2014 Oct 10;5:5212
Nature communications 2014 Oct 10;5:5212
Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.
Agarwal AR, Zhao L, Sancheti H, Sundar IK, Rahman I, Cadenas E
American journal of physiology. Lung cellular and molecular physiology 2012 Nov 15;303(10):L889-98
American journal of physiology. Lung cellular and molecular physiology 2012 Nov 15;303(10):L889-98
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15932-1-AP(IDH2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 15932-1-AP(IDH2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The IDH2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IDH2. This antibody recognizes human,mouse,rat antigen. The IDH2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
