15932-1-AP

antibody from Proteintech Group

Targeting: IDH2

Provider product page for 15932-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [45]
Comments [0]
Validations
Western blot [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

Submit

Product number: 15932-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15932-1-AP, RRID:AB_2264612
Product name: IDH2 antibody
Antibody type: Polyclonal
Description: KD/KO validated IDH2 antibody (Cat. #15932-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IDH2 protein structure - 15932-1-AP shown in red.

Submitted references Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats.

Li D, Yao H, Ren Y, Shang J, Han X, Cao X, Song T, Zeng X
General and comparative endocrinology 2024 Mar 1;348:114448

D-mannose promotes the degradation of IDH2 through upregulation of RNF185 and suppresses breast cancer.

Zhang R, Tian Z, Xu Y, Lv L
Nutrition & metabolism 2024 Jan 2;21(1):5

LINC00571 drives tricarboxylic acid cycle metabolism in triple-negative breast cancer through HNRNPK/ILF2/IDH2 axis.

Xi Z, Huang H, Hu J, Yu Y, Ma X, Xu M, Ming J, Li L, Zhang H, Chen H, Huang T
Journal of experimental & clinical cancer research : CR 2024 Jan 18;43(1):22

Protein lysine acetylation played an important role in NH(3)-induced AEC2 damage and pulmonary fibrosis in piglets.

Wang X, Zhang D, Zhu Y, Li D, Shen L, Wang Q, Gao Y, Li X, Yu M
The Science of the total environment 2024 Jan 15;908:168303

Targeting PRMT1 prevents acute and chronic graft-versus-host disease.

Zhao X, Sun Y, Xu Z, Cai L, Hu Y, Wang H
Molecular therapy : the journal of the American Society of Gene Therapy 2023 Nov 1;31(11):3259-3276

Sirt3-dependent regulation of mitochondrial oxidative stress and apoptosis contributes to the dysfunction of pancreatic islets after severe burns.

Liu X, Xie X, Li D, Liu Z, Zhang B, Zang Y, Yuan H, Shen C
Free radical biology & medicine 2023 Mar;198:59-67

Oncogenic IDH mutations increase heterochromatin-related replication stress without impacting homologous recombination.

Schvartzman JM, Forsyth G, Walch H, Chatila W, Taglialatela A, Lee BJ, Zhu X, Gershik S, Cimino FV, Santella A, Menghrajani K, Ciccia A, Koche R, Sánchez-Vega F, Zha S, Thompson CB
Molecular cell 2023 Jul 6;83(13):2347-2356.e8

The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.

Bassot A, Chen J, Takahashi-Yamashiro K, Yap MC, Gibhardt CS, Le GNT, Hario S, Nasu Y, Moore J, Gutiérrez T, Mina L, Mast H, Moses A, Bhat R, Ballanyi K, Lemieux H, Sitia R, Zito E, Bogeski I, Campbell RE, Simmen T
Cell reports 2023 Jan 31;42(1):111899

Formononetin attenuates high glucose-induced neurotoxicity by negatively regulating oxidative stress and mitochondrial dysfunction in Schwann cells via activation of SIRT3.

Jiang W, Hu T, Ye C, Hu M, Yu Q, Sun L, Liang J, Chen Y
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Dec;182:114156

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma.

Hu Q, Xu J, Wang L, Yuan Y, Luo R, Gan M, Wang K, Zhao T, Wang Y, Han T, Wang JB
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Dec;10(35):e2303535

Astaxanthin promotes mitochondrial biogenesis and antioxidant capacity in chronic high-intensity interval training.

Wang Y, Chen X, Baker JS, Davison GW, Xu S, Zhou Y, Bao X
European journal of nutrition 2023 Apr;62(3):1453-1466

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

Terado T, Kim CJ, Ushio A, Minami K, Tambe Y, Kageyama S, Kawauchi A, Tsunoda T, Shirasawa S, Tanaka H, Inoue H
International journal of oncology 2022 Sep;61(3)

Changes in the Expression of MIF and Other Key Enzymes of Energy Metabolism in the Myocardia of Broiler Chickens with Ascites Syndrome.

Li L, Jia Q, Chen L, Wang W
Animals : an open access journal from MDPI 2022 Sep 20;12(19)

SIRT5 alleviates hepatic ischemia and reperfusion injury by diminishing oxidative stress and inflammation via elevating SOD1 and IDH2 expression.

Zheng D, Qiwen Zeng, He D, He Y, Yang J
Experimental cell research 2022 Oct 15;419(2):113319

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.

Yuan M, Wang Y, Wen J, Jing F, Zou Q, Pu Y, Pan T, Cai Z
Aging and disease 2022 Oct 1;13(5):1532-1545

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Wang Q, Wu M, Li H, Rao X, Ao L, Wang H, Yao L, Wang X, Hong X, Wang J, Aa J, Sun M, Wang G, Liu J, Zhou F
Pharmacological research 2022 Nov;185:106490

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Fan TW, Daneshmandi S, Cassel TA, Uddin MB, Sledziona J, Thompson PT, Lin P, Higashi RM, Lane AN
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690

MUC1 triggers lineage plasticity of Her2 positive mammary tumors.

Pang Z, Dong X, Deng H, Wang C, Liao X, Liao C, Liao Y, Tian W, Cheng J, Chen G, Yi H, Huang L
Oncogene 2022 May;41(22):3064-3078

Non-oxidative pentose phosphate pathway controls regulatory T cell function by integrating metabolism and epigenetics.

Liu Q, Zhu F, Liu X, Lu Y, Yao K, Tian N, Tong L, Figge DA, Wang X, Han Y, Li Y, Zhu Y, Hu L, Ji Y, Xu N, Li D, Gu X, Liang R, Gan G, Wu L, Zhang P, Xu T, Hu H, Hu Z, Xu H, Ye D, Yang H, Li B, Tong X
Nature metabolism 2022 May;4(5):559-574

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Zhang S, Huang W, Ren L, Ju X, Gong M, Rao J, Sun L, Li P, Ding Q, Wang J, Zhang QC
Cell research 2022 Jan;32(1):9-23

The mTOR/PGC-1α/SIRT3 Pathway Drives Reductive Glutamine Metabolism to Reduce Oxidative Stress Caused by ISKNV in CPB Cells.

Fu X, Li K, Niu Y, Lin Q, Liang H, Luo X, Liu L, Li N
Microbiology spectrum 2022 Feb 23;10(1):e0231021

Global profiling of protein lysine malonylation in mouse cardiac hypertrophy.

Wu LF, Wang DP, Shen J, Gao LJ, Zhou Y, Liu QH, Cao JM
Journal of proteomics 2022 Aug 30;266:104667

Mandarin Fish (Siniperca chuatsi) p53 Regulates Glutaminolysis Induced by Virus via the p53/miR145-5p/c-Myc Pathway in Chinese Perch Brain Cells.

Ye C, Liu S, Li N, Zuo S, Niu Y, Lin Q, Liang H, Luo X, Fu X
Microbiology spectrum 2022 Apr 27;10(2):e0272721

Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome.

Peoples JN, Ghazal N, Duong DM, Hardin KR, Manning JR, Seyfried NT, Faundez V, Kwong JQ
American journal of physiology. Cell physiology 2021 Sep 1;321(3):C519-C534

Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.

Pang JL, Huang FH, Zhang YH, Wu Y, Ge XM, Li S, Li X
Toxicology and applied pharmacology 2021 Nov 1;430:115726

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794

Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.

Chen X, Zhuo S, Xu W, Chen X, Huang D, Sun X, Cheng Y
British journal of cancer 2020 Jul;123(1):126-136

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

Oberhuber M, Pecoraro M, Rusz M, Oberhuber G, Wieselberg M, Haslinger P, Gurnhofer E, Schlederer M, Limberger T, Lagger S, Pencik J, Kodajova P, Högler S, Stockmaier G, Grund-Gröschke S, Aberger F, Bolis M, Theurillat JP, Wiebringhaus R, Weiss T, Haitel A, Brehme M, Wadsak W, Griss J, Mohr T, Hofer A, Jäger A, Pollheimer J, Egger G, Koellensperger G, Mann M, Hantusch B, Kenner L
Molecular systems biology 2020 Apr;16(4):e9247

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H, Maimouni S, Dai W, Velasquez S, Pan JA, Muthalagu N, Morton J, Anthony TG, Feng H, Lamers WH, Murphy DJ, Guo JY, Jin J, Crawford HC, Zhang L, White E, Lin RZ, Su X, Tuveson DA, Zong WX
Cell reports 2019 Oct 29;29(5):1287-1298.e6

Two human patient mitochondrial pyruvate carrier mutations reveal distinct molecular mechanisms of dysfunction.

Oonthonpan L, Rauckhorst AJ, Gray LR, Boutron AC, Taylor EB
JCI insight 2019 May 30;5(13)

Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.

Ju R, Fei K, Li S, Chen C, Zhu L, Li J, Zhang D, Guo L, Ye C
The Journal of pharmacology and experimental therapeutics 2018 Oct;367(1):20-27

Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.

White K, Kim MJ, Han C, Park HJ, Ding D, Boyd K, Walker L, Linser P, Meneses Z, Slade C, Hirst J, Santostefano K, Terada N, Miyakawa T, Tanokura M, Salvi R, Someya S
Scientific reports 2018 Mar 22;8(1):5039

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Wu L, Cao KX, Ni ZH, Li WD, Chen ZP, Cheng HB, Liu X
Journal of ethnopharmacology 2018 Aug 10;222:71-78

Exogenous H(2)S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Sun Y, Tian Z, Liu N, Zhang L, Gao Z, Sun X, Yu M, Wu J, Yang F, Zhao Y, Ren H, Chen H, Zhao D, Wang Y, Dong S, Xu C, Lu F, Zhang W
Journal of molecular medicine (Berlin, Germany) 2018 Apr;96(3-4):281-299

The clinical significance of isocitrate dehydrogenase 2 in esophageal squamous cell carcinoma.

Chen X, Xu W, Wang C, Liu F, Guan S, Sun Y, Wang X, An D, Wen Z, Chen P, Cheng Y
American journal of cancer research 2017;7(3):700-714

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825

Loss of NAD-Dependent Protein Deacetylase Sirtuin-2 Alters Mitochondrial Protein Acetylation and Dysregulates Mitophagy.

Liu G, Park SH, Imbesi M, Nathan WJ, Zou X, Zhu Y, Jiang H, Parisiadou L, Gius D
Antioxidants & redox signaling 2017 May 20;26(15):849-863

Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.

Zang S, Tang Q, Dong F, Liu H, Li L, Guo F, Pan X, Lin H, Zeng W, Cai Z, Zhong Q, Zang N, Zang L
Oncology reports 2017 Dec;38(6):3583-3591

SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.

Zou X, Zhu Y, Park SH, Liu G, O'Brien J, Jiang H, Gius D
Cancer research 2017 Aug 1;77(15):3990-3999

On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel.

Heo H, Kim S, Lee HH, Cho HR, Xu WJ, Lee SH, Park CK, Park S, Choi SH, Kim H
PloS one 2016;11(1):e0147794

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831

MiR-181a regulates lipid metabolism via IDH1.

Chu B, Wu T, Miao L, Mei Y, Wu M
Scientific reports 2015 Mar 5;5:8801

Metabolomic comparison between cells over-expressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism.

Wen H, Cho HR, Yun T, Kim H, Park CK, Lee SH, Choi SH, Park S
Journal of neurochemistry 2015 Jan;132(2):183-93

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Ma X, Li C, Sun L, Huang D, Li T, He X, Wu G, Yang Z, Zhong X, Song L, Gao P, Zhang H
Nature communications 2014 Oct 10;5:5212

Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs.

Agarwal AR, Zhao L, Sancheti H, Sundar IK, Rahman I, Cadenas E
American journal of physiology. Lung cellular and molecular physiology 2012 Nov 15;303(10):L889-98

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15932-1-AP(IDH2 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15932-1-AP(IDH2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IDH2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IDH2. This antibody recognizes human,mouse,rat antigen. The IDH2 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
Appendix: 15932-1-AP.pdf

: Show more