Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-43572 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IDH2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IDH2 antibody dilution: 1:1000
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [T673].
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [673] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Mouse kidney [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min