Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003418-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003418-M01, RRID:AB_539741
- Product name
- Mouse Monoclonal IDH2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. IDH2 - isocitrate dehydrogenase 2 (NADP+), mitochondrial
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.
Decreased expression of ten-eleven translocation 2 protein is associated with progressive disease and death in patients with mycosis fungoides.
Altered global methylation and hydroxymethylation status in vulvar lichen sclerosus: further support for epigenetic mechanisms.
The effect of dietary soy isoflavones before and after ovariectomy on hippocampal protein markers of mitochondrial bioenergetics and antioxidant activity in female monkeys.
Ferreira JCB, Campos JC, Qvit N, Qi X, Bozi LHM, Bechara LRG, Lima VM, Queliconi BB, Disatnik MH, Dourado PMM, Kowaltowski AJ, Mochly-Rosen D
Nature communications 2019 Jan 18;10(1):329
Nature communications 2019 Jan 18;10(1):329
Decreased expression of ten-eleven translocation 2 protein is associated with progressive disease and death in patients with mycosis fungoides.
Gambichler T, Mamali K, Patsinakidis N, Moritz R, Mucke M, Skrygan M, Stockfleth E, Stücker M
The British journal of dermatology 2016 Mar;174(3):652-3
The British journal of dermatology 2016 Mar;174(3):652-3
Altered global methylation and hydroxymethylation status in vulvar lichen sclerosus: further support for epigenetic mechanisms.
Gambichler T, Terras S, Kreuter A, Skrygan M
The British journal of dermatology 2014 Mar;170(3):687-93
The British journal of dermatology 2014 Mar;170(3):687-93
The effect of dietary soy isoflavones before and after ovariectomy on hippocampal protein markers of mitochondrial bioenergetics and antioxidant activity in female monkeys.
Rettberg JR, Hamilton RT, Mao Z, To J, Zhao L, Appt SE, Register TC, Kaplan JR, Brinton RD
Brain research 2011 Mar 16;1379:23-33
Brain research 2011 Mar 16;1379:23-33
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IDH2 Antibody (5F11) [H00003418-M01] - Analysis of IDH2 expression in transfected 293T cell line by IDH2 monoclonal antibody (M01), clone 5F11.Lane 1: IDH2 transfected lysate(50.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: IDH2 Antibody (5F11) [H00003418-M01] - Detection limit for recombinant GST tagged IDH2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IDH2 Antibody (5F11) [H00003418-M01] - Analysis of monoclonal antibody to IDH2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
