PA5-96605

antibody from Invitrogen Antibodies

Targeting: IDH2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96605

Provider

Invitrogen Antibodies

Product name

IDH2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KMVFTPKDGS GVKEWEVYNF PAGGVGMGMY NTDESISGFA HSCFQYAIQK KWPLYMSTKN TILKAYDGRF KDIFQEIFDK HYKTDFDKNK IWYEHRLIDD MVAQVLKSSG GFVWACKNYD GDVQSDILAQ GFGSLGLMTS VLVCPDGKTI EAEAAHGTVT RHYREHQKGR PTSTNPIASI FAWTRGLEHR GKLDGNQDLI RFAQMLEKVC VETVESGAMT KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDRALGRQ; Positive Samples: HL-60, MCF-7, Jurkat, HepG2, U-87MG, Mouse kidney, Mouse heart, Rat lung, Rat kidney; Cellular Location: Mitochondrion

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.65 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IDH2 was performed in C6 cells using IDH2 Polyclonal Antibody (Product # PA5-96605).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IDH2 was performed in L929 cells using IDH2 Polyclonal Antibody (Product # PA5-96605).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of IDH2 in NIH/3T3 cells. Samples were incubated with IDH2 Polyclonal antibody (Product # PA5-96605) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of IDH2 in 200 μg extracts of MCF7 cells. Samples were precipitated with 1 μg IDH2 Polyclonal antibody (Product # PA5-96605). Western blot was performed from the immunoprecipitate using IDH2 Polyclonal antibody (Product # PA5-96605) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IDH2 in paraffin-embedded human liver cancer. Samples were incubated with IDH2 Polyclonal antibody (Product # PA5-96605) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of IDH2 in 293T cell line using IDH2 Polyclonal Antibody (Product # PA5-96605) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of IDH2 in extracts of 293T cell line. Samples were incubated with IDH2 Polyclonal antibody (Product # PA5-96605) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of IDH2 was performed in 200 µg extracts of MCF7 cells using IDH2 Polyclonal Antibody (Product # PA5-96605). Western blot was performed from the immunoprecipitate using IDH2 Polyclonal Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of IDH2 was performed in 200 µg extracts of MCF7 cells using IDH2 Polyclonal Antibody (Product # PA5-96605). Western blot was performed from the immunoprecipitate using IDH2 Polyclonal Antibody.