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antibody from Invitrogen Antibodies
Targeting: IDH2
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-96605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KMVFTPKDGS GVKEWEVYNF PAGGVGMGMY NTDESISGFA HSCFQYAIQK KWPLYMSTKN TILKAYDGRF KDIFQEIFDK HYKTDFDKNK IWYEHRLIDD MVAQVLKSSG GFVWACKNYD GDVQSDILAQ GFGSLGLMTS VLVCPDGKTI EAEAAHGTVT RHYREHQKGR PTSTNPIASI FAWTRGLEHR GKLDGNQDLI RFAQMLEKVC VETVESGAMT KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDRALGRQ; Positive Samples: HL-60, MCF-7, Jurkat, HepG2, U-87MG, Mouse kidney, Mouse heart, Rat lung, Rat kidney; Cellular Location: Mitochondrion
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH2 in extracts of various cell lines using IDH2 Polyclonal Antibody (Product # PA5-96605) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IDH2 was performed in C6 cells using IDH2 Polyclonal Antibody (Product # PA5-96605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IDH2 was performed in L929 cells using IDH2 Polyclonal Antibody (Product # PA5-96605).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IDH2 in paraffin-embedded human colon carcinoma using IDH2 Polyclonal Antibody (Product # PA5-96605) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of IDH2 in 293T cell line using IDH2 Polyclonal Antibody (Product # PA5-96605) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IDH2 was performed in 200 µg extracts of MCF7 cells using IDH2 Polyclonal Antibody (Product # PA5-96605). Western blot was performed from the immunoprecipitate using IDH2 Polyclonal Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IDH2 was performed in 200 µg extracts of MCF7 cells using IDH2 Polyclonal Antibody (Product # PA5-96605). Western blot was performed from the immunoprecipitate using IDH2 Polyclonal Antibody.
