Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX628487 - Provider product page
- Provider
- GeneTex
- Product name
- IDH2 antibody [GT673]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
Submitted references Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis.
Liu TF, Vachharajani V, Millet P, Bharadwaj MS, Molina AJ, McCall CE
The Journal of biological chemistry 2015 Jan 2;290(1):396-408
The Journal of biological chemistry 2015 Jan 2;290(1):396-408
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody [GT673] detects IDH2 protein by Western blot analysis.A. 50 µg rat brain lysate/extract12 % SDS-PAGEIDH2 antibody [GT673] (GTX628487) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody detects IDH2 protein by Western blot analysis.A. 30 µg Jurkat whole cell lysate/extractB. 30 µg Raji whole cell lysate/extractC. 30 µg K562 whole cell lysate/extractD. 30 µg THP-1 whole cell lysate/extractE. 30 µg NCI-H929 whole cell lysate/extract10 % SDS-PAGEIDH2 antibody (GTX628487) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody [GT673] detects IDH2 protein by Western blot analysis.A. 50 µg mouse brain lysate/extract10 % SDS-PAGEIDH2 antibody [GT673] (GTX628487) dilution: 1:1000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500.Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH2 antibody detects IDH2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. IDH2 antibody (GTX628487) dilution: 1:100.
